Table 3.
Disulfide bond SS1 (Cys10-Cys24) peptides.
| Assignment | Theoretical Mass (Da) | Observed Mass (Da) | Mass Error (Da) | Intensity (counts) | Identification | ||
|---|---|---|---|---|---|---|---|
| 30 Fragments | Constituent Peptides 10 Fragments | 1/a2 | 173.129 | 173.0568 | 0.0722 | 38 | VT |
| 1/b5 | 516.267 | 516.2606 | 0.0063 | 69 | VTVDT | ||
| 1/y1 | 147.1133 | 147.0415 | 0.0718 | 19 | K | ||
| 2/a2 | 177.1028 | 177.1026 | 0.0002 | 567 | GF | ||
| 2/b2 | 205.0977 | 205.0983 | −0.0006 | 691 | GF | ||
| 2/b3 | 318.1818 | 318.1831 | −0.0013 | 634 | GFL | ||
| 2/b4 | 431.2658 | 431.1857 | 0.0801 | 82 | GFLI | ||
| 2/b6 | 690.3649 | 690.3612 | 0.0037 | 40 | GFLIQM | ||
| 2/b7 | 777.3969 | 777.4837 | −0.0868 | 34 | GFLIQMS | ||
| 2/b9 | 971.4773 | 971.4425 | 0.0348 | 52 | GFLIQMSGH | ||
| Cys10 and Cys24 20 Fragments | 1/b7-2/y3 | 1094.486 | 1094.477 | 0.0095 | 103 | VTVDTVC=ECK | |
| 1/b7-2/y9 | 1747.782 | 1747.827 | −0.0454 | 86 | VTVDTVC=QMSGHLECK | ||
| 1/y2-2/a12 | 1535.717 | 1535.675 | 0.0422 | 196 | CK=GFLIQMSGHLEC | ||
| 1/y2-2/y4 | 739.3483 | 739.3598 | −0.0115 | 163 | CK=LECK | ||
| 1/y2-2/y5 | 876.4072 | 876.3971 | 0.0101 | 68 | CK=HLECK | ||
| 1/y2-2/z4 | 722.3217 | 722.2222 | 0.0995 | 26 | CK=LECK | ||
| 1/y3-2/y3 | 725.3326 | 725.3351 | −0.0025 | 101 | VCK=ECK | ||
| 1/y3-2/y4 | 838.4167 | 838.3221 | 0.0946 | 56 | VCK=LECK | ||
| 1/y4-2/y12 | 1852.912 | 1852.852 | 0.0603 | 119 | TVCK=FLIQMSGHLECK | ||
| 1/y5-2/y3 | 941.4072 | 941.3948 | 0.0125 | 50 | DTVCK=ECK | ||
| 1/y5-2/y5 | 1191.55 | 1191.505 | 0.0454 | 74 | DTVCK=HLECK | ||
| 1/y5-2/y7 | 1335.604 | 1335.515 | 0.0884 | 7251 | DTVCK=SGHLECK | ||
| 1/y5-2/y8 | 1466.644 | 1466.662 | −0.0175 | 177 | DTVCK=MSGHLECK | ||
| 1/y7-2/y10 | 1907.903 | 1907.823 | 0.0797 | 268 | TVDTVCK=IQMSGHLECK | ||
| 1/y7-2/y2 | 1012.481 | 1012.438 | 0.0428 | 181 | TVDTVCK=CK | ||
| 1/y8-2/y10 | 2006.971 | 2006.947 | 0.0239 | 390 | VTVDTVCK=IQMSGHLECK | ||
| 1/y8-2/y11 | 2120.055 | 2120.046 | 0.0098 | 112 | VTVDTVCK=LIQMSGHLECK | ||
| 1/y8-2/y13 | 2324.145 | 2324.142 | 0.0029 | 4580 | VTVDTVCK=GFLIQMSGHLECK | ||
| 1/y8-2/z10 | 1989.945 | 1989.963 | −0.0178 | 63 | VTVDTVCK=IQMSGHLECK | ||
| 1/z7-2/y2 | 995.4542 | 995.5009 | −0.0468 | 47 | TVDTVCK=CK | ||