Table 6.
Disulfide bond SS4 (Cys54-Cys72) peptides.
| Assignment | Theoretical Mass (Da) | Observed Mass (Da) | Mass Error (Da) | Intensity (counts) | Identification | ||
|---|---|---|---|---|---|---|---|
| 68 Fragments | Constituent Peptides 23 Fragments | 1/a2 | 173.129 | 173.1297 | −0.0007 | 4507 | TV |
| 1/a3 | 287.1719 | 287.0969 | 0.075 | 897 | TVN | ||
| 1/a4 | 415.2669 | 415.2224 | 0.0445 | 28 | TVNK | ||
| 1/b2 | 201.1239 | 201.1235 | 0.0004 | 2754 | TV | ||
| 1/b3 | 315.1668 | 315.0896 | 0.0772 | 51 | TVN | ||
| 1/b4 | 443.2618 | 443.2626 | −0.0008 | 248 | TVNK | ||
| 1/y1 | 147.1133 | 147.0623 | 0.051 | 16 | K | ||
| 1/y2 | 234.1454 | 234.1455 | −0.0001 | 700 | SK | ||
| 1/y3 | 381.2138 | 381.2014 | 0.0124 | 1146 | FSK | ||
| 1/y4 | 496.2407 | 496.2404 | 0.0003 | 153 | DFSK | ||
| 1/y5 | 553.2622 | 553.2604 | 0.0018 | 564 | GDFSK | ||
| 2/a3 | 258.1454 | 258.1545 | −0.0092 | 78 | IDG | ||
| 2/a4 | 372.1883 | 372.1927 | −0.0044 | 300 | IDGN | ||
| 2/a5 | 469.2411 | 469.2278 | 0.0132 | 76 | IDGNP | ||
| 2/a6 | 568.3094 | 568.3065 | 0.0029 | 91 | IDGNPV | ||
| 2/a8 | 818.4048 | 818.3653 | 0.0396 | 129 | IDGNPVSY | ||
| 2/b1 | 114.0919 | 114.041 | 0.0509 | 18 | I | ||
| 2/b2 | 229.1188 | 229.119 | −0.0002 | 1962 | ID | ||
| 2/b3 | 286.1403 | 286.1415 | −0.0012 | 727 | IDG | ||
| 2/b4 | 400.1832 | 400.1889 | −0.0057 | 1203 | IDGN | ||
| 2/b5 | 497.236 | 497.2373 | −0.0013 | 252 | IDGNP | ||
| 2/b6 | 596.3044 | 596.3082 | −0.0038 | 205 | IDGNPV | ||
| 2/b7 | 683.3364 | 683.3206 | 0.0158 | 211 | IDGNPVS | ||
| Cys54 and Cys72 45 Fragments | 1/a6-2/y5 | 1183.5604 | 1183.541 | 0.0198 | 1608 | TVNKPC=SYACK | |
| 1/a6-2/y7 | 1379.6815 | 1379.604 | 0.0773 | 143 | TVNKPC=PVSYACK | ||
| 1/a8-2/y7 | 1551.73 | 1551.666 | 0.0636 | 108 | TVNKPCGD=PVSYACK | ||
| 1/a9-2/b10 | 1951.8682 | 1951.823 | 0.0454 | 93 | TVNKPCGDF=IDGNPVSYAC | ||
| 1/b10-2/a10 | 2038.9003 | 2038.99 | −0.09 | 43 | TVNKPCGDFS=IDGNPVSYAC | ||
| 1/b6-2/y3 | 961.4599 | 961.4065 | 0.0534 | 124 | TVNKPC=ACK | ||
| 1/b6-2/y8 | 1521.7194 | 1521.674 | 0.0449 | 161 | TVNKPC=NPVSYACK | ||
| 1/b7-2/y7 | 1464.6979 | 1464.675 | 0.0228 | 47 | TVNKPCG=PVSYACK | ||
| 1/b8-2/y10 | 1865.8162 | 1865.827 | −0.0106 | 68 | TVNKPCGD=DGNPVSYACK | ||
| 1/b8-2/y11 | 1978.9003 | 1978.891 | 0.0092 | 579 | TVNKPCGD= IDGNPVSYACK | ||
| 1/b8-2/y2 | 1062.4712 | 1062.441 | 0.0303 | 364 | TVNKPCGD=CK | ||
| 1/b8-2/y6 | 1482.6721 | 1482.651 | 0.021 | 137 | TVNKPCGD=VSYACK | ||
| 1/b8-2/y7 | 1579.7249 | 1579.69 | 0.0347 | 456 | TVNKPCGD=PVSYACK | ||
| 1/b9-2/y5 | 1530.6721 | 1530.667 | 0.0054 | 180 | TVNKPCGDF=SYACK | ||
| 1/b9-2/y7 | 1726.7932 | 1726.741 | 0.0527 | 66 | TVNKPCGDF=PVSYACK | ||
| 1/y11-2/y10 | 2246.0222 | 2246.022 | 0.0005 | 2740 | TVNKPCGDFSK=DGNPVSYACK | ||
| 1/y11-2/y11 | 2359.1062 | 2359.109 | −0.0027 | 57983 | TVNKPCGDFSK= IDGNPVSYACK | ||
| 1/y11-2/y2 | 1442.6771 | 1442.674 | 0.0034 | 677 | TVNKPCGDFSK=CK | ||
| 1/y11-2/y3 | 1513.7142 | 1513.711 | 0.0028 | 849 | TVNKPCGDFSK=ACK | ||
| 1/y11-2/y4 | 1676.7776 | 1676.777 | 0.0009 | 999 | TVNKPCGDFSK=YACK | ||
| 1/y11-2/y5 | 1763.8097 | 1763.806 | 0.0037 | 1320 | TVNKPCGDFSK=SYACK | ||
| 1/y11-2/y6 | 1862.8781 | 1862.893 | −0.0144 | 201 | TVNKPCGDFSK=VSYACK | ||
| 1/y11-2/y7 | 1959.9308 | 1959.926 | 0.005 | 6462 | TVNKPCGDFSK=PVSYACK | ||
| 1/y11-2/y9 | 2130.9951 | 2131.013 | −0.0173 | 418 | TVNKPCGDFSK=GNPVSYACK | ||
| 1/y11-2/z10 | 2228.9956 | 2228.999 | −0.0037 | 1038 | TVNKPCGDFSK=DGNPVSYACK | ||
| 1/y6-2/a10 | 1645.699 | 1645.775 | −0.0762 | 368 | CGDFSK=IDGNPVSYAC | ||
| 1/y6-2/y11 | 1819.7994 | 1819.795 | 0.0044 | 57 | CGDFSK= IDGNPVSYACK | ||
| 1/y6-2/y4 | 1137.4708 | 1137.472 | −0.0007 | 738 | CGDFSK=YACK | ||
| 1/y6-2/y7 | 1420.624 | 1420.613 | 0.0114 | 404 | CGDFSK=PVSYACK | ||
| 1/y7-2/b10 | 1770.7467 | 1770.722 | 0.0243 | 43 | PCGDFSK= IDGNPVSYAC | ||
| 1/y7-2/y11 | 1916.8523 | 1916.848 | 0.0039 | 3031 | PCGDFSK= IDGNPVSYACK | ||
| 1/y7-2/y2 | 1000.4232 | 1000.424 | −0.0004 | 627 | PCGDFSK=CK | ||
| 1/y7-2/y3 | 1071.4603 | 1071.461 | −0.0007 | 825 | PCGDFSK=ACK | ||
| 1/y7-2/y5 | 1321.5557 | 1321.558 | −0.0024 | 1193 | PCGDFSK=SYACK | ||
| 1/y7-2/y7 | 1517.6769 | 1517.681 | −0.0043 | 1667 | PCGDFSK=PVSYACK | ||
| 1/y7-2/y9 | 1688.7412 | 1688.747 | −0.0057 | 547 | PCGDFSK=GNPVSYACK | ||
| 1/y8-2/y11 | 2044.9471 | 2044.929 | 0.0182 | 64 | KPCGDFSK= IDGNPVSYACK | ||
| 1/y8-2/y2 | 1128.5182 | 1128.502 | 0.0162 | 491 | KPCGDFSK=CK | ||
| 1/y8-2/y4 | 1362.6187 | 1362.648 | −0.0288 | 120 | KPCGDFSK=YACK | ||
| 1/y8-2/y5 | 1449.6506 | 1449.647 | 0.0038 | 253 | KPCGDFSK=SYACK | ||
| 1/y8-2/y8 | 1759.8147 | 1759.8 | 0.0151 | 186 | KPCGDFSK=NPVSYACK | ||
| 1/y9-2/y11 | 2158.9902 | 2158.979 | 0.0112 | 1071 | NKPCGDFSK=IDGNPVSYACK | ||
| 1/y9-2/y3 | 1313.5981 | 1313.58 | 0.0184 | 279 | NKPCGDFSK=ACK | ||
| 1/y9-2/y4 | 1476.6615 | 1476.644 | 0.0173 | 144 | NKPCGDFSK=YACK | ||
| 1/z7-2/y2 | 983.3967 | 983.4453 | −0.0486 | 113 | PCGDFSK=CK | ||