Table 7.
Disulfide bond SS5 (Cys74-Cys85) and SS6 (Cys90-Cys100) peptides.
| Assignment | Theoretical Mass (Da) | Observed Mass (Da) | Mass Error (Da) | Intensity (counts) | Identification | ||
|---|---|---|---|---|---|---|---|
| 90 Fragments | Cys74 and Cys85 4 Fragments | 1/b12 | 1324.513 | 1324.516 | −0.0031 | 1267 | CNLGYDMVNNVC (Internal) |
| 1/b13 | 1437.597 | 1437.596 | 0.0006 | 3004 | CNLGYDMVNNVCI (Internal) | ||
| 1/b15 | 1648.692 | 1648.71 | −0.0175 | 311 | CNLGYDMVNNVCIPN (Internal) | ||
| 1/b16 | 1777.735 | 1777.728 | 0.007 | 105 | CNLGYDMVNNVCIPNE (Internal) | ||
| Constituent Peptides 9 Fragments | 1/y1 | 147.1133 | 147.1123 | 0.001 | 2902 | K | |
| 2/y2 | 246.1818 | 246.1842 | −0.0024 | 789 | VK | ||
| 2/y3 | 343.2345 | 343.1509 | 0.0836 | 258 | PVK | ||
| 2/y4 | 457.2774 | 457.2771 | 0.0003 | 855 | NPVK | ||
| 2/y5 | 544.3095 | 544.3134 | −0.0039 | 711 | SNPVK | ||
| 2/y6 | 645.3572 | 645.3581 | −0.0009 | 3696 | TSNPVK | ||
| 2/y7 | 760.3841 | 760.3795 | 0.0046 | 2330 | DTSNPVK | ||
| 2/y8 | 873.4681 | 873.4631 | 0.0051 | 2651 | LDTSNPVK | ||
| 2/y9 | 986.5522 | 986.5494 | 0.0029 | 381 | ILDTSNPVK | ||
| Cys90-Cys100 33 fragments | 1/a17-2/a7 | 2569.065 | 2569.078 | −0.0127 | 433 | CNLGYDMVNNVCIPNEC=CILDTSN | |
| 1/y18-2/b2 | 2240.927 | 2240.983 | −0.0564 | 114 | CNLGYDMVNNVCIPNECK=CI | ||
| 1/y18-2/b4 | 2469.038 | 2469.068 | −0.03 | 1446 | CNLGYDMVNNVCIPNECK=CILD | ||
| 1/y18-2/b6 | 2657.118 | 2657.106 | 0.012 | 90 | CNLGYDMVNNVCIPNECK=CILDTS | ||
| 1/y2-2/a8 | 1063.492 | 1063.406 | 0.0859 | 167 | CK=CILDTSNP | ||
| 1/y2-2/b1 | 351.1161 | 351.204 | −0.0879 | 78 | CK=C | ||
| 1/y2-2/b2 | 464.2001 | 464.2095 | −0.0094 | 359 | CK=CI | ||
| 1/y2-2/b3 | 577.2842 | 577.284 | 0.0002 | 142 | CK=CIL | ||
| 1/y2-2/b4 | 692.3112 | 692.2997 | 0.0115 | 322 | CK=CILD | ||
| 1/y2-2/b7 | 994.4338 | 994.4278 | 0.006 | 837 | CK=CILDTSN | ||
| 1/y2-2/y10 | 1336.661 | 1336.634 | 0.027 | 664 | CK=CILDTSNPVK | ||
| 1/y3-2/b1 | 480.1587 | 480.1505 | 0.0081 | 107 | ECK=C | ||
| 1/y3-2/b4 | 821.3538 | 821.3419 | 0.0118 | 460 | ECK=CILD | ||
| 1/y3-2/b5 | 922.4014 | 922.3771 | 0.0244 | 283 | ECK=CILDT | ||
| 1/y3-2/b8 | 1220.529 | 1220.519 | 0.0098 | 340 | ECK=CILDTSNP | ||
| 1/y3-2/b9 | 1319.598 | 1319.584 | 0.0138 | 987 | ECK=CILDTSNPV | ||
| 1/y4-2/a1 | 566.2067 | 566.2282 | −0.0215 | 232 | NECK=C | ||
| 1/y4-2/a2 | 679.2908 | 679.3111 | −0.0203 | 202 | NECK=CI | ||
| 1/y4-2/a7 | 1209.524 | 1209.502 | 0.0221 | 927 | NECK=CILDTSN | ||
| 1/y4-2/b3 | 820.3697 | 820.3667 | 0.003 | 154 | NECK=CIL | ||
| 1/y4-2/b4 | 935.3967 | 935.3924 | 0.0043 | 481 | NECK=CILD | ||
| 1/y4-2/b5 | 1036.444 | 1036.448 | −0.0039 | 191 | NECK=CILDT | ||
| 1/y4-2/b8 | 1334.572 | 1334.572 | 0 | 1655 | NECK=CILDTSNP | ||
| 1/y4-2/y10 | 1579.746 | 1579.675 | 0.0706 | 5505 | NECK=CILDTSNPVK | ||
| 1/y5-2/b8 | 1431.625 | 1431.599 | 0.0256 | 644 | PNECK=CILDTSNP | ||
| 1/y5-2/y10 | 1676.799 | 1676.798 | 0.0012 | 15020 | PNECK=CILDTSNPVK | ||
| 1/y6-2/a1 | 776.3435 | 776.3327 | 0.0108 | 842 | IPNECK=C | ||
| 1/y6-2/a6 | 1305.618 | 1305.539 | 0.0798 | 217 | IPNECK=CILDTS | ||
| 1/y6-2/b2 | 917.4225 | 917.4044 | 0.0181 | 1033 | IPNECK=CI | ||
| 1/y6-2/b3 | 1030.507 | 1030.413 | 0.0941 | 390 | IPNECK=CIL | ||
| 1/y6-2/y10 | 1789.883 | 1789.867 | 0.0161 | 2137 | IPNECK=CILDTSNPVK | ||
| 1/z4-2/y10 | 1562.72 | 1562.661 | 0.0585 | 2069 | NECK=CILDTSNPVK | ||
| 1/z5-2/y10 | 1659.772 | 1659.854 | −0.0822 | 2401 | PNECK=CILDTSNPVK | ||
| Cys74 to Cys85 and Cys90 to Cys100 44 Fragments | 1/y10-1/a1–2/a2 | 1392.59 | 1392.579 | 0.0105 | 789 | NVCIPNECK=C=CI | |
| 1/y10-2/b9-1/a5 | 2594.151 | 2594.122 | 0.0295 | 85 | NVCIPNECK=CILDTSNPV=CNLGY | ||
| 1/y11-1/a4-2/a4 | 2003.918 | 2003.83 | 0.0873 | 148 | VNNVCIPNECK=CNLG=CILD | ||
| 1/y11-1/b4-2/b1 | 1718.712 | 1718.686 | 0.0265 | 115 | VNNVCIPNECK=CNLG=C | ||
| 1/y11-1/b6-2/b6 | 2526.077 | 2525.993 | 0.084 | 186 | VNNVCIPNECK=CNLGYD=CILDTS | ||
| 1/y12-2/a2-1/a5 | 2069.91 | 2069.88 | 0.0305 | 554 | MVNNVCIPNECK=CI=CNLGY | ||
| 1/y12-2/b5-1/a2 | 2093.895 | 2093.833 | 0.0625 | 298 | MVNNVCIPNECK=CILDT=CN | ||
| 1/y13-1/b1–2/a9 | 2492.075 | 2492.059 | 0.0166 | 490 | DMVNNVCIPNECK=C=CILDTSNPV | ||
| 1/y13-1/b2-2/a3 | 1992.847 | 1992.854 | −0.0063 | 826 | DMVNNVCIPNECK=CN=CIL | ||
| 1/y13-2/b3-1/b4 | 2190.948 | 2190.909 | 0.0388 | 313 | DMVNNVCIPNECK=CIL=CNLG | ||
| 1/y13-2/b5-1/a2 | 2208.922 | 2208.972 | −0.0498 | 102 | DMVNNVCIPNECK=CILDT=CN | ||
| 1/y14-1/a1–2/a8 | 2528.075 | 2527.986 | 0.0891 | 572 | YDMVNNVCIPNECK=C=CILDTSNP | ||
| 1/y14-2/b9-1/a3 | 2882.266 | 2882.24 | 0.0254 | 1679 | YDMVNNVCIPNECK=CILDTSNPV=CNL | ||
| 1/y15-1/a1–2/a9 | 2684.165 | 2684.074 | 0.0908 | 230 | GYDMVNNVCIPNECK=C=CILDTSNPV | ||
| 1/y15-1/b2-2/b9 | 2854.198 | 2854.166 | 0.0322 | 311 | GYDMVNNVCIPNECK=CN=CILDTSNPV | ||
| 1/y7-1/b1–2/b2 | 1121.425 | 1121.524 | −0.0988 | 3609 | CIPNECK=C=CI | ||
| 1/y7-2/a1-1/a2 | 1066.394 | 1066.391 | 0.0034 | 286 | CIPNECK=C=CN | ||
| 1/y7-2/a1-1/a8 | 1744.699 | 1744.779 | −0.0803 | 1284 | CIPNECK=C=CNLGYDMV | ||
| 1/y7-2/a2-1/a7 | 1758.715 | 1758.619 | 0.0957 | 156 | CIPNECK=CI=CNLGYDM | ||
| 1/y7-2/a3-1/a9 | 2084.91 | 2084.817 | 0.0925 | 164 | CIPNECK=CIL=CNLGYDMVN | ||
| 1/y7-2/a5-1/a3 | 1621.721 | 1621.642 | 0.0795 | 207 | CIPNECK=CILDT=CNL | ||
| 1/y7-2/a9-1/a11 | 2911.292 | 2911.285 | 0.0068 | 205 | CIPNECK=CILDTSNPV=CNLGYDMVNNV | ||
| 1/y7-2/a9-1/a8 | 2584.138 | 2584.076 | 0.062 | 990 | CIPNECK=CILDTSNPV=CNLGYDMV | ||
| 1/y7-2/b1-1/a3 | 1207.473 | 1207.5 | −0.027 | 508 | CIPNECK=C=CNL | ||
| 1/y7-2/b1-1/a6 | 1542.585 | 1542.666 | −0.0806 | 928 | CIPNECK=C=CNLGYD | ||
| 1/y7-2/b2-1/b4 | 1405.574 | 1405.526 | 0.0474 | 125 | CIPNECK=CI=CNLG | ||
| 1/y7-2/b3-1/b4 | 1518.658 | 1518.643 | 0.015 | 197 | CIPNECK=CIL=CNLG | ||
| 1/y7-2/b4-1/a10 | 2341.975 | 2341.988 | −0.0132 | 340 | CIPNECK=CILD=CNLGYDMVNN | ||
| 1/y7-2/b6-1/a10 | 2530.054 | 2529.985 | 0.0696 | 102 | CIPNECK=CILDTS=CNLGYDMVNN | ||
| 1/y7-2/b8-1/b3 | 1975.839 | 1975.88 | −0.0417 | 245 | CIPNECK=CILDTSNP=CNL | ||
| 1/y8-1/a1–2/a5 | 1493.663 | 1493.629 | 0.0336 | 182 | VCIPNECK=C=CILDT | ||
| 1/y8-2/b9-1/a3 | 2145.981 | 2145.931 | 0.0496 | 164 | VCIPNECK=CILDTSNPV=CNL | ||
| 1/y9-1/b1–2/b5 | 1663.695 | 1663.662 | 0.0337 | 104 | NVCIPNECK=C=CILDT | ||
| 1/y9-2/b1-1/a6 | 1755.696 | 1755.718 | −0.0212 | 658 | NVCIPNECK=C=CNLGYD | ||
| 1/y9-2/b1-1/b1 | 1221.453 | 1221.521 | −0.068 | 782 | NVCIPNECK=C=C | ||
| 1/y9-2/b8-1/a6 | 2496.067 | 2496.051 | 0.0159 | 634 | NVCIPNECK=CILDTSNP=CNLGYD | ||
| 1/y9-2/b9-1/a3 | 2260.023 | 2259.99 | 0.0334 | 668 | NVCIPNECK=CILDTSNPV=CNL | ||
| 2/y10-1/y12-1/b2 | 2665.192 | 2665.097 | 0.0942 | 258 | CILDTSNPVK=MVNNVCIPNECK=CN | ||
| 2/y10-1/y7-1/b10 | 3014.319 | 3014.31 | 0.0088 | 1407 | CILDTSNPVK=CIPNECK=CNLGYDMVNN | ||
| 2/y10-1/y7-1/b11 | 3113.388 | 3113.393 | −0.0056 | 120269 | CILDTSNPVK=CIPNECK=CNLGYDMVNNV | ||
| 2/y10-1/y7-1/b6 | 2556.124 | 2556.095 | 0.0291 | 1309 | CILDTSNPVK=CIPNECK=CNLGYD | ||
| 2/y10-1/y9-1/a4 | 2463.15 | 2463.093 | 0.0574 | 609 | CILDTSNPVK=NVCIPNECK=CNLG | ||
| 2/y10-1/y9-1/a5 | 2626.214 | 2626.114 | 0.0999 | 654 | CILDTSNPVK=NVCIPNECK=CNLGY | ||
| 2/y10-1/y9-1/b3 | 2434.124 | 2434.059 | 0.0645 | 571 | CILDTSNPVK=NVCIPNECK=CNL | ||