Table 8.
Disulfide bond SS7 (Cys95-Cys113) and SS8 (Cys115-Cys129) peptides.
| Assignment | Theoretical Mass (Da) | Observed Mass (Da) | Mass Error (Da) | Intensity (counts) | Identification | ||
|---|---|---|---|---|---|---|---|
| 39 Fragments | Constituent Peptides 11 Fragments | 1/a2 | 200.1399 | 200.141 | −0.0011 | 247 | QV |
| 1/a3 | 301.1876 | 301.1464 | 0.0412 | 100 | QVT | ||
| 1/b1 | 129.0664 | 129.0653 | 0.0011 | 33 | Q | ||
| 1/y1 | 147.1133 | 147.1145 | −0.0012 | 1258 | K | ||
| 1/y3 | 318.1777 | 318.1789 | −0.0012 | 47 | NGK | ||
| 1/y4 | 375.1992 | 375.2 | −0.0008 | 52 | GNGK | ||
| 2/a2 | 131.082 | 131.0274 | 0.0546 | 30 | T | ||
| 2/b1 | 102.0555 | 102.0558 | −0.0003 | 107 | T | ||
| 2/b2 | 159.077 | 159.0768 | 0.0002 | 140 | TG | ||
| 2/y2 | 204.1348 | 204.0705 | 0.0643 | 30 | GK | ||
| 2/y4 | 431.2618 | 431.2624 | −0.0006 | 72 | NIGK | ||
| Cys95 to Cys113 3 Fragments | 1/b7-2/b5 | 1105.441 | 1105.43 | 0.0109 | 157 | QVTCGNG=TGVCS | |
| 1/y6-2/a4 | 909.3923 | 909.4301 | −0.0378 | 58 | TCGNGK=TGVC | ||
| 1/y6-2/b4 | 937.3871 | 937.3987 | −0.0115 | 257 | TCGNGK=TGVC | ||
| Cys115 to Cys129 4 Fragments | 2/y5-3/b2 | 722.2966 | 722.3025 | −0.0059 | 45 | CNIGK=CS | |
| 2/y5-3/y3 | 868.4021 | 868.3544 | 0.0477 | 103 | CNIGK=CSK | ||
| 2/y6-3/a2 | 781.3337 | 781.4172 | −0.0835 | 21 | SCNIGK=CS | ||
| 2/y6-3/b2 | 809.3286 | 809.3259 | 0.0027 | 90 | SCNIGK=CS | ||
| T12, T14, and T16 linkage 21 Fragments | 1/a4-2/a6-3/a1 | 997.3728 | 997.3982 | −0.0254 | 39 | QVTC=TGVCSC=C | |
| 1/a4-2/a6-3/a2 | 1084.405 | 1084.434 | −0.0294 | 146 | QVTC=TGVCSC=CS | ||
| 1/a4-2/a8-3/b2 | 1339.527 | 1339.585 | −0.0582 | 210 | QVTC=TGVCSCNI=CS | ||
| 1/a4-2/y8-3/y3 | 1558.685 | 1558.64 | 0.0449 | 111 | QVTC=VCSCNIGK=CSK | ||
| 1/a5-2/a6-3/a1 | 1054.394 | 1054.432 | −0.0376 | 60 | QVTCG=TGVCSC=C | ||
| 1/a6-2/y8-3/y3 | 1729.749 | 1729.66 | 0.089 | 51 | QVTCGN=VCSCNIGK=CSK | ||
| 1/a7-2/a7-3/b1 | 1367.497 | 1367.472 | 0.0244 | 33 | QVTCGNG=TGVCSCN=C | ||
| 1/b5-2/a9-3/b2 | 1481.565 | 1481.493 | 0.0713 | 35 | QVTCG=TGVCSCNIG=CS | ||
| 1/b6-2/a7-3/b1 | 1338.47 | 1338.53 | −0.0596 | 72 | QVTCGN=TGVCSCN=C | ||
| 1/b6-2/b9-3/y3 | 1769.708 | 1769.651 | 0.0566 | 1155 | QVTCGN=TGVCSCNIG=CSK | ||
| 1/y5-2/b6-3/y3 | 1360.512 | 1360.561 | −0.0487 | 249 | CGNGK=TGVCSC=CSK | ||
| 1/y5-2/b9-3/b1 | 1411.523 | 1411.599 | −0.0762 | 348 | CGNGK=TGVCSCNIG=C | ||
| 1/y5-2/y8-3/a1 | 1371.564 | 1371.529 | 0.035 | 254 | CGNGK=VCSCNIGK=C | ||
| 1/y5-2/y8-3/b2 | 1486.591 | 1486.645 | −0.0536 | 270 | CGNGK=VCSCNIGK=CS | ||
| 1/y6-2/y10-3/y3 | 1891.814 | 1891.761 | 0.0521 | 105 | TCGNGK=TGVCSCNIGK=CSK | ||
| 1/y7-2/b8-3/b2 | 1641.649 | 1641.631 | 0.0188 | 78 | VTCGNGK=TGVCSCNI=CS | ||
| 1/y7-2/y10-3/y3 | 1990.882 | 1990.798 | 0.0837 | 124 | VTCGNGK=TGVCSCNIGK=CSK | ||
| 1/y7-2/y8-3/b2 | 1686.707 | 1686.75 | −0.0424 | 6211 | VTCGNGK=VCSCNIGK=CS | ||
| 1/y8-2/b8-3/b1 | 1682.676 | 1682.723 | −0.0466 | 32 | QVTCGNGK=TGVCSCNI=C | ||
| 1/y8-2/b9-3/y3 | 1972.835 | 1972.819 | 0.0156 | 407 | QVTCGNGK=TGVCSCNIG=CSK | ||
| 1/y8-2/y10-3/y3 | 2118.94 | 2118.932 | 0.0085 | 4955 | QVTCGNGK=TGVCSCNIGK=CSK | ||