Table 9.
Disulfide bond SS9 (Cys137-Cys148) peptides.
| Assignment | Theoretical Mass (Da) | Observed Mass (Da) | Mass Error (Da) | Intensity (counts) | Identification | ||
|---|---|---|---|---|---|---|---|
| 35 Fragments | constituent peptides 10 Fragments | 1/y2 | 260.1974 | 260.2041 | −0.0067 | 262 | LK |
| 1/y3 | 347.2294 | 347.2299 | −0.0005 | 129 | SLK | ||
| 2/a1 | 102.0555 | 102.0559 | −0.0004 | 754 | E | ||
| 2/a2 | 216.0984 | 216.1371 | −0.0387 | 65 | EN | ||
| 2/a4 | 446.1887 | 446.2249 | −0.0362 | 20 | ENET | ||
| 2/b1 | 130.0504 | 130.0469 | 0.0035 | 17 | E | ||
| 2/b2 | 244.0933 | 244.0929 | 0.0004 | 367 | EN | ||
| 2/b3 | 373.1359 | 373.1376 | −0.0017 | 171 | ENE | ||
| 2/b4 | 474.1836 | 474.1958 | −0.0122 | 88 | ENET | ||
| 2/y1 | 147.1133 | 147.1133 | 0 | 1131 | K | ||
| Cys137 and Cys148 25 Fragments | 1/a1–2/y2 | 323.1212 | 323.1008 | 0.0204 | 15 | C=CK | |
| 1/a1–2/y4 | 553.2114 | 553.2058 | 0.0056 | 53 | C=ETCK | ||
| 1/a1–2/y5 | 667.2544 | 667.2748 | −0.0204 | 59 | C=NETCK | ||
| 1/a2-2/y2 | 410.1532 | 410.1623 | −0.0091 | 36 | CS=CK | ||
| 1/a2-2/y4 | 640.2435 | 640.2652 | −0.0217 | 34 | CS=ETCK | ||
| 1/a3-2/y3 | 624.2849 | 624.2893 | −0.0044 | 27 | CSL=CKT | ||
| 1/a3-2/y5 | 867.3705 | 867.3508 | 0.0197 | 89 | CSL=NETCK | ||
| 1/b1–2/a5 | 650.1914 | 650.2377 | −0.0463 | 130 | C=ENETC | ||
| 1/b2-2/a5 | 737.2234 | 737.2545 | −0.0311 | 139 | CS=ENETC | ||
| 1/b1–2/y2 | 351.1161 | 351.1717 | −0.0556 | 207 | C=CK | ||
| 1/b1–2/y3 | 452.1638 | 452.1826 | −0.0188 | 23 | C=CKT | ||
| 1/b2-2/y2 | 438.1481 | 438.1512 | −0.0031 | 139 | CS=CK | ||
| 1/b2-2/y3 | 539.1958 | 539.2008 | −0.005 | 166 | CS=CKT | ||
| 1/b2-2/y5 | 782.2813 | 782.3434 | −0.0621 | 180 | CS=NETCK | ||
| 1/b3-2/y2 | 551.2322 | 551.2324 | −0.0002 | 219 | CSL=CK | ||
| 1/y4-2/y2 | 697.3377 | 697.3329 | 0.0048 | 465 | CSLK=CK | ||
| 1/y4-2/a5 | 996.413 | 996.4069 | 0.0061 | 130 | CSLK=ENETC | ||
| 1/y4-2/b5 | 1024.408 | 1024.4126 | −0.0046 | 502 | CSLK=ENETC | ||
| 1/y4-2/y3 | 798.3854 | 798.3802 | 0.0052 | 553 | CSLK=TCK | ||
| 1/y4-2/y4 | 927.428 | 927.4216 | 0.0064 | 223 | CSLK=ETCK | ||
| 1/y4-2/y5 | 1041.4709 | 1041.4377 | 0.0332 | 94 | CSLK=NETCK | ||
| 1/y4-2/y6 | 1170.5134 | 1170.511 | 0.0024 | 25002 | CSLK=ENETCK | ||
| 1/a1–2/y6 | 796.2969 | 796.3787 | −0.0818 | 646 | C=ENETCK | ||
| 1/b2-2/y6 | 911.3239 | 911.2963 | 0.0276 | 155 | CS=ENETCK | ||
| 1/y4-2/z3 | 781.3588 | 781.3229 | 0.0359 | 77 | CSLK=TCK | ||