Table 10.
Disulfide bond SS10 (Cys141-Cys157) and SS11 (Cys159-Cys172) peptides.
| Assignment | Theoretical Mass (Da) | Observed Mass (Da) | Mass Error (Da) | Intensity (counts) | Identification | ||
|---|---|---|---|---|---|---|---|
| 65 Fragments | Constituent Peptides 19 fragments | 1/y1 | 147.1133 | 147.1126 | 0.0007 | 1153 | K |
| 1/y2 | 260.1974 | 260.2021 | −0.0047 | 167 | LK | ||
| 3/a2 | 145.0613 | 145.0646 | −0.0033 | 400 | DG | ||
| 3/a5 | 518.2979 | 518.296 | 0.0018 | 109 | DGFII | ||
| 3/a8 | 890.426 | 890.3521 | 0.0739 | 84 | DGFIIDQE | ||
| 3/a9 | 977.458 | 977.3666 | 0.0914 | 120 | DGFIIDQES | ||
| 3/b1 | 116.0348 | 116.0532 | −0.0184 | 28 | D | ||
| 3/b2 | 173.0562 | 173.0561 | 0.0001 | 645 | DG | ||
| 3/b3 | 320.1246 | 320.1256 | −0.001 | 6533 | DGF | ||
| 3/b4 | 433.2087 | 433.2064 | 0.0023 | 1906 | DGFI | ||
| 3/b5 | 546.2927 | 546.2884 | 0.0043 | 325 | DGFII | ||
| 3/b6 | 661.3197 | 661.3064 | 0.0133 | 295 | DGFIID | ||
| 3/b9 | 1005.453 | 1005.383 | 0.0699 | 237 | DGFIIDQES | ||
| 3/y1 | 156.0773 | 156.0769 | 0.0004 | 4578 | H | ||
| 3/y2 | 293.1362 | 293.1368 | −0.0006 | 1684 | HH | ||
| 3/y3 | 430.1951 | 430.1913 | 0.0038 | 1430 | HHH | ||
| 3/y4 | 567.254 | 567.2552 | −0.0012 | 1460 | HHHH | ||
| 3/y5 | 704.3129 | 704.3094 | 0.0035 | 1025 | HHHHH | ||
| 3/y7 | 942.4196 | 942.4053 | 0.0143 | 568 | THHHHHH | ||
| Cys141 to Cys157 11 Fragments | 1/a1–2/a1 | 149.0207 | 149.1165 | −0.0958 | 16 | C=C | |
| 1/a2-2/a1 | 262.1048 | 262.1324 | −0.0276 | 39 | CL=C | ||
| 1/a2-2/a2 | 377.1317 | 377.1469 | −0.0152 | 258 | CL=CD | ||
| 1/a2-2/b1 | 290.0997 | 290.1243 | −0.0246 | 45 | CL=C | ||
| 1/a2-2/b2 | 405.1266 | 405.1262 | 0.0005 | 230 | CL=CD | ||
| 1/y3-2/a2 | 551.2322 | 551.2063 | 0.0259 | 62 | CLK=CD | ||
| 1/b1–2/a2 | 292.0426 | 292.0312 | 0.0114 | 82 | C=CD | ||
| 1/b1–2/b1 | 205.0106 | 205.0966 | −0.086 | 154 | C=C | ||
| 1/b2-2/b1 | 318.0946 | 318.0872 | 0.0074 | 36 | CL=C | ||
| 1/y3-2/b1 | 464.2001 | 464.214 | −0.0139 | 41 | CLK=C | ||
| 1/y3-2/b2 | 579.2271 | 579.2289 | −0.0018 | 1027 | CLK=CD | ||
| Cys159 to Cys172 3 fragments | 2/y2–3/a12 | 1527.682 | 1527.653 | 0.0291 | 242 | CK=DGFIIDQESSIC | |
| 2/y2–3/y19 | 2497.089 | 2497.066 | 0.0227 | 994 | CK=DGFIIDQESSICTHHHHHH | ||
| 2/y3-3/a12 | 1642.709 | 1642.633 | 0.0763 | 69 | DCK=DGFIIDQESSIC | ||
| T12, T14, and T16 Linkage 32 Fragments | 1/a1–2/a3-3/a16 | 2156.8411 | 2156.815 | 0.0266 | 37 | C=CDC=DGFIIDQESSICTHHH | |
| 1/a1–2/a3-3/y10 | 1609.5782 | 1609.559 | 0.0193 | 132 | C=CDC=SICTHHHHHH | ||
| 1/a1–2/a3-3/y14 | 2068.7383 | 2068.799 | −0.0608 | 74 | C=CDC=DQESSICTHHHHHH | ||
| 1/a1–2/y4-3/y18 | 2673.0967 | 2673.159 | −0.0623 | 62 | C=CDCK=GFIIDQESSICTHHHHHH | ||
| 1/a1–2/y4-3/y8 | 1583.5625 | 1583.616 | −0.0533 | 363 | C=CDCK=CTHHHHHH | ||
| 1/a2-2/a3-3/a16 | 2269.925 | 2269.92 | 0.0051 | 921 | CL=CDC=DGFIIDQESSICTHHH | ||
| 1/a2-2/a3-3/a18 | 2544.043 | 2544.035 | 0.0076 | 58 | CL=CDC=DGFIIDQESSICTHHHHH | ||
| 1/a2-2/y4-3/a15 | 2306.9666 | 2306.904 | 0.0623 | 147 | CL=CDCK=DGFIIDQESSICTHH | ||
| 1/b1–2/b3-3/a12 | 1700.6064 | 1700.657 | −0.0505 | 100 | C=CDC=DGFIIDQESSIC | ||
| 1/b1–2/b3-3/y14 | 2124.7283 | 2124.776 | −0.0476 | 72 | C=CDC=DQESSICTHHHHHH | ||
| 1/b1–2/y4-3/a14 | 2084.8186 | 2084.862 | −0.0437 | 85 | C=CDCK=DGFIIDQESSICTH | ||
| 1/b1–2/y4-3/y17 | 2644.0703 | 2644.117 | −0.0471 | 2248 | C=CDCK=FIIDQESSICTHHHHHH | ||
| 1/b2-2/b3-3/a12 | 1813.6906 | 1813.709 | −0.0181 | 63 | CL=CDC=DGFIIDQESSIC | ||
| 1/b2-2/b3-3/b13 | 1942.7332 | 1942.833 | −0.0997 | 95 | CL=CDC=DGFIIDQESSICT | ||
| 1/b2-2/b3-3/y8 | 1578.536 | 1578.608 | −0.0721 | 228 | CL=CDC=CTHHHHHH | ||
| 1/b2-2/b3-3/y9 | 1691.62 | 1691.693 | −0.0729 | 105 | CL=CDC=ICTHHHHHH | ||
| 1/b2-2/y4-3/a14 | 2197.9026 | 2197.909 | −0.0066 | 671 | CL=CDCK=DGFIIDQESSICTH | ||
| 1/b2-2/y4-3/b17 | 2637.0742 | 2637.017 | 0.0576 | 233 | CL=CDCK=DGFIIDQESSICTHHHH | ||
| 1/b2-2/y4-3/y10 | 1924.7576 | 1924.81 | −0.0524 | 84 | CL=CDCK=SICTHHHHHH | ||
| 1/b2-2/y4-3/y16 | 2610.0859 | 2610.088 | −0.0017 | 407 | CL=CDCK=IIDQESSICTHHHHHH | ||
| 1/y3-2/y4-3/a18 | 2892.2439 | 2892.164 | 0.0803 | 403 | CLK=CDCK=DGFIIDQESSICTHHHHH | ||
| 1/y3-2/y4-3/b14 | 2372.0032 | 2372.038 | −0.0352 | 341 | CLK=CDCK=DGFIIDQESSICTH | ||
| 1/y3-2/y4-3/b18 | 2920.2388 | 2920.248 | −0.0088 | 1160 | CLK=CDCK=GFIIDQESSICTHHHHHH | ||
| 1/y3-2/y4-3/y11 | 2157.8953 | 2157.893 | 0.0024 | 922 | CLK=CDCK=SSICTHHHHHH | ||
| 1/y3-2/y4-3/y13 | 2414.9963 | 2415.007 | −0.011 | 897 | CLK=CDCK=QESSICTHHHHHH | ||
| 1/y3-2/y4-3/y14 | 2530.0232 | 2530.028 | −0.0046 | 2415 | CLK=CDCK=DQESSICTHHHHHH | ||
| 1/y3-2/y4-3/y16 | 2756.1914 | 2756.2 | −0.0088 | 1421 | CLK=CDCK=IIDQESSICTHHHHHH | ||
| 1/y3-2/y4-3/y18 | 2960.2812 | 2960.238 | 0.043 | 911 | CLK=CDCK=GFIIDQESSICTHHHHHH | ||
| 1/y3-2/y4-3/y19 | 3075.3081 | 3075.308 | 0.0005 | 42471 | CLK=CDCK=DGFIIDQESSICTHHHHHH | ||
| 1/y3-2/y4-3/y8 | 1870.7471 | 1870.768 | −0.0212 | 131 | CLK=CDCK=CTHHHHHH | ||
| 1/y3-2/y4-3/z14 | 2512.9968 | 2513.063 | −0.0657 | 102 | CLK=CDCK=DQESSICTHHHHHH | ||
| 1/y3-2/y4-3/z8 | 1853.7205 | 1853.793 | −0.0724 | 66 | CLK=CDCK=CTHHHHHH | ||