Subject area	Chemistry, Biology,
More specific subject area	Disulfide bond analysis by liquid chromatography and mass spectrometric analysis
Type of data	Tables, figures
How data was acquired	Data was generated using liquid chromatography (Waters 2695 Separations Module and Waters 2489 UV/Vis Detector) and mass spectroscopy (Waters QTOF Premier mass spectrometer)
Data format	Collated data from analysis with Waters BiopharmaLynx 1.3 and MassLynx
Experimental factors	Recombinant Pfs25 digested with 20 µg of trypsin/Lys-C at 37 °C overnight and subsequent further digestion by additional 20 µg of trypsin/Lys-C for 3–4 hours at 37 °C
Experimental features	Identification of the proper pairing of 11 disulfide bonds in Pfs25 through digestion of peptides and LC-MS/MS
Data source location	Mass spectrometry data acquired in Middleton, WI, USA
Data accessibility	Data is provided within this article and RAW MS files have been deposited in the Mass Spectrometry Interactive Virtual Environment (MassIVE) with identifier: MSV000081982 (ftp://massive.ucsd.edu/MSV000081982). MassIVE is a member of the ProteomeXchange Consortium