Table 1.
TAG profiles of control, DHA- and sPLA2-treated MDA-MB-231 breast cancer cells.
|
TAG composition (% of total)a | |||
|---|---|---|---|
| TAG speciesb | Untreatedc | DHA | DHA+sPLA2 |
| 46:0 | 0.36 ± 0.03 | n.d. | 0.05 ± 0.02 |
| 48:0 | 1.2 ± 0.1 | 0.07 ± 0.01 | 0.16 ± 0.03 |
| 48:1 | 1.3 ± 0.1 | 0.12 ± 0.01 | 0.2 ± 0.1 |
| 50:0 | 3.3 ± 0.4 | 0.14 ± 0.02 | 0.4 ± 0.1 |
| 50:1 | 5.5 ± 0.1 | 0.4 ± 0.1 | 1.0 ± 0.2 |
| 50:2 | 2.3 ± 0.2 | 0.2 ± 0.1 | 0.6 ± 0.1 |
| 52:0 | 6.0 ± 1.0 | 0.26 ± 0.04 | 0.8 ± 0.1 |
| 52:1 | 11.3 ± 0.3 | 1.1 ± 0.1 | 1.8 ± 0.3 |
| 52:2 | 8.1 ± 0.2 | 1.0 ± 0.1 | 2.0 ± 1.0 |
| 52:3 | 3.0 ± 0.3 | 0.44 ± 0.03 | 0.9 ± 0.2 |
| 52:4 | 0.64 ± 0.03 | 0.15 ± 0.01 | 0.3 ± 0.1 |
| 54:0 | 1.8 ± 0.1 | 0.06 ± 0.01 | 0.17 ± 0.01 |
| 54:1 | 8.2 ± 0.3 | 0.72 ± 0.02 | 1.3 ± 0.2 |
| 54:2 | 6.0 ± 1.0 | 0.7 ± 0.1 | 1.3 ± 0.1 |
| 54:3 | 5.0 ± 1.0 | 0.8 ± 0.1 | 1.7 ± 0.4 |
| 54:4 | 2.9 ± 0.1 | 0.6 ± 0.1 | 1.1 ± 0.2 |
| 54:5 | 2.4 ± 0.1 | n.d. | n.d. |
| 54:6 | 1.2 ± 0.1 | 2.6 ± 0.4 | 2.6 ± 0.2 |
| 54:7 | 0.22 ± 0.03 | 1.1 ± 0.1 | 1.26 ± 0.03 |
| 56:0 | 1.0 ± 0.1 | n.d. | 0.10 ± 0.01 |
| 56:1 | 1.85 ± 0.02 | 0.16 ± 0.03 | 0.23 ± 0.03 |
| 56:2 | 1.8 ± 0.1 | 0.26 ± 0.02 | 0.4 ± 0.1 |
| 56:3 | 1.6 ± 0.1 | 0.28 ± 0.02 | 0.43 ± 0.03 |
| 56:6 | 4.1 ± 0.3 | 3.0 ± 1.0 | 5.4 ± 0.4 |
| 56:7 | 1.2 ± 0.2 | 4.4 ± 0.3 | 6.7 ± 0.1 |
| 58:1 | 1.9 ± 0.1 | 0.120 ± 0.004 | 0.25 ± 0.01 |
| 58:2 | 1.19 ± 0.02 | 0.12 ± 0.02 | 0.23 ± 0.03 |
| 58:3 | 0.6 ± 0.1 | n.d. | 0.17 ± 0.02 |
| 58:5 | 1.6 ± 0.2 | 0.17 ± 0.02 | 0.7 ± 0.1 |
| 58:6 | 2.5 ± 0.2 | n.d. | n.d. |
| 58:7 | 1.7 ± 0.2 | 4.6 ± 0.4 | 7.2 ± 0.2 |
| 58:8 | 0.5 ± 0.1 | 2.8 ± 0.2 | 4.8 ± 0.1 |
| 58:9 | 0.12 ± 0.02 | 1.00 ± 0.03 | 1.7 ± 0.1 |
| 58:10 | n.d. | 0.83 ± 0.02 | 1.3 ± 0.1 |
| 58:11 | 0.02 ± 0.01 | 0.91 ± 0.04 | 1.1 ± 0.1 |
| 58:12 | 0.03 ± 0.02 | 3.1 ± 0.3 | 1.9 ± 0.2 |
| 60:1 | 2.21 ± 0.04 | n.d. | 0.31 ± 0.04 |
| 60:2 | 1.47 ± 0.03 | 0.146 ± 0.002 | 0.31 ± 0.02 |
| 60:3 | 0.5 ± 0.1 | 0.04 ± 0.01 | 0.16 ± 0.01 |
| 60:4 | 0.32 ± 0.04 | 0.05 ± 0.02 | 0.12 ± 0.02 |
| 60:10 | 0.07 ± 0.01 | 1.91 ± 0.02 | 3.0 ± 0.2 |
| 60:12 | 0.8 ± 0.1 | 18.0 ± 1.0 | 14.1 ± 1.1 |
| 60:13 | n.d. | 3.4 ± 0.1 | 1.6 ± 0.3 |
| 62:1 | 0.9 ± 0.1 | n.d. | n.d. |
| 62:2 | 0.79 ± 0.03 | 0.05 ± 0.01 | 0.163 ± 0.003 |
| 62:3 | 0.40 ± 0.02 | 0.027 ± 0.002 | 0.112 ± 0.004 |
| 62:11 | n.d. | 1.1 ± 0.2 | 1.5 ± 0.2 |
| 62:12 | 0.5 ± 0.1 | 9.0 ± 0.3 | 9.0 ± 1.0 |
| 62:13 | 0.8 ± 0.1 | 15.0 ± 1.0 | 14.0 ± 1.0 |
| 62:14 | n.d. | 2.9 ± 0.1 | 2.2 ± 0.2 |
| 66:18 | 0.3 ± 0.1 | 15.0 ± 2.0 | 7.0 ± 1.0 |
a
TAG composition data is presented as mean percentages of each species per total detected species and normalised to total cellular protein.
b
TAG species are described based on the number of carbon atoms and double bonds.
c
MDA-MB-231 cells were treated with 100 µM DHA and 10 nM sPLA2 in complete medium for 48 h and cell lysates were collected for UPLC/MS analysis. Values are means ± SEM of three independent experiments; n.d., not determined.