Table 2.
PC profiles of control, DHA- and sPLA2-treated MDA-MB-231 breast cancer cells.
|
PC composition (% of total)a | |||
|---|---|---|---|
| PC speciesb | Untreatedc | DHA | DHA+sPLA2 |
| 30:0 | 2.7 ± 0.2 | 4.8 ± 0.3 | 3.2 ± 0.2 |
| 30:1 | 2.3 ± 0.2 | 2.3 ± 0.1 | 2.4 ± 0.2 |
| 32:0 | 5.0 ± 0.1 | 21.0 ± 0.3 | 13.0 ± 1.0 |
| 32:1 | 9.0 ± 1.0 | 7.0 ± 1.0 | 6.1 ± 0.3 |
| 34:1 | 28.0 ± 1.0 | 17.0 ± 1.0 | 23.0 ± 1.0 |
| 34:2 | 5.3 ± 0.3 | 2.9 ± 0.2 | 3.1 ± 0.1 |
| 34:3 | 0.26 ± 0.01 | 1.1 ± 0.1 | 0.6 ± 0.1 |
| 36:0 | 0.47 ± 0.04 | 0.31 ± 0.02 | 0.6 ± 0.1 |
| 36:1 | 21.0 ± 1.0 | 7.9 ± 0.3 | 15.0 ± 1.0 |
| 36:2 | 13.0 ± 0.1 | 4.9 ± 0.1 | 7.6 ± 0.2 |
| 36:4 | 2.46 ± 0.04 | 2.65 ± 0.01 | 2.9 ± 0.1 |
| 36:5 | 0.48 ± 0.04 | 1.4 ± 0.1 | 0.9 ± 0.1 |
| 36:6 | 0.07 ± 0.01 | 1.0 ± 0.2 | 0.29 ± 0.01 |
| 38:3 | 1.9 ± 0.1 | 1.1 ± 0.1 | 1.5 ± 0.1 |
| 38:4 | 3.0 ± 0.1 | 2.6 ± 0.3 | 3.4 ± 0.4 |
| 38:5 | 2.0 ± 0.3 | 2.7 ± 0.2 | 3.1 ± 0.1 |
| 38:6 | 1.1 ± 0.1 | 9.0 ± 1.0 | 6.0 ± 1.0 |
| 40:6 | 1.5 ± 0.1 | 7.0 ± 1.0 | 6.0 ± 1.0 |
| 40:7 | 0.5 ± 0.1 | 3.1 ± 0.3 | 2.0 ± 0.3 |
a
PC composition data is presented as mean percentages of each species per total detected species and normalised to total cellular protein.
b
PC species are described based on the number of carbon atoms and double bonds.
c
MDA-MB-231 cells were treated with 100 µM DHA and 10 nM sPLA2 in complete medium for 48 h and cell lysates were collected for UPLC/MS analysis. Values are means ± SEM of three independent experiments; n.d., not determined.