Table 3.
TAG profiles of MDA-MB-231 breast cancer cells depleted in ATGL and treated with exogenous DHA, sPLA2 or both.
|
TAG composition (% of total)a |
||||||
|---|---|---|---|---|---|---|
| TAG speciesb | SCRc | siATGL | SCR+DHA | siATGL+DHA | SCR+DHA+sPLA2 | siATGL+DHA+sPLA2 |
| 46:0 | 0.30 ± 0.03 | 0.42 ± 0.02 | 0.031 ± 0.003 | 0.05 ± 0.01 | 0.03 ± 0.01 | 0.03 ± 0.01 |
| 48:0 | 1.3 ± 0.1 | 1.9 ± 0.1 | 0.11 ± 0.02 | 0.16 ± 0.01 | 0.08 ± 0.01 | 0.14 ± 0.01 |
| 48:1 | 1.19 ± 0.02 | 1.5 ± 0.1 | 0.16 ± 0.02 | 0.26 ± 0.04 | 0.15 ± 0.03 | 0.17 ± 0.03 |
| 50:0 | 3.8 ± 0.3 | 5.41 ± 0.03 | 0.3 ± 0.1 | 0.5 ± 0.1 | 0.2 ± 0.1 | 0.5 ± 0.1 |
| 50:1 | 5.1 ± 0.2 | 6.8 ± 0.1 | 0.5 ± 0.1 | 1.1 ± 0.2 | 0.6 ± 0.1 | 0.7 ± 0.1 |
| 50:2 | 1.94 ± 0.04 | 2.4 ± 0.2 | 0.3 ± 0.1 | 0.6 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.1 |
| 52:0 | 6.3 ± 0.4 | 7.9 ± 0.2 | 0.5 ± 0.1 | 0.9 ± 0.1 | 0.4 ± 0.1 | 0.75 ± 0.02 |
| 52:1 | 11.0 ± 1.0 | 12.0 ± 1.0 | 1.1 ± 0.2 | 2.2 ± 0.3 | 1.1 ± 0.2 | 1.6 ± 0.1 |
| 52:2 | 8.0 ± 1.0 | 8.9 ± 0.4 | 1.4 ± 0.1 | 2.1 ± 0.3 | 1.3 ± 0.2 | 1.4 ± 0.2 |
| 52:3 | 2.3 ± 0.2 | 2.7 ± 0.2 | 0.6 ± 0.1 | 0.7 ± 0.1 | 0.5 ± 0.1 | 0.4 ± 0.1 |
| 52:4 | 0.68 ± 0.03 | 0.7 ± 0.1 | 0.20 ± 0.03 | 0.21 ± 0.02 | 0.21 ± 0.03 | 0.19 ± 0.02 |
| 54:0 | 1.7 ± 0.2 | 1.75 ± 0.03 | 0.09 ± 0.01 | 0.2 ± 0.1 | 0.07 ± 0.01 | 0.1327 ± 0.0002 |
| 54:1 | 8.1 ± 0.4 | 8.0 ± 1.0 | 0.7 ± 0.1 | 1.3 ± 0.2 | 0.7 ± 0.1 | 1.2 ± 0.2 |
| 54:2 | 5.5 ± 0.3 | 5.4 ± 0.2 | 0.6 ± 0.1 | 1.3 ± 0.2 | 0.9 ± 0.1 | 1.02 ± 0.01 |
| 54:3 | 5.0 ± 1.0 | 5.0 ± 1.0 | 1.1 ± 0.1 | 1.5 ± 0.2 | 1.2 ± 0.1 | 0.98 ± 0.01 |
| 54:4 | 2.5 ± 0.2 | 2.3 ± 0.1 | 0.6 ± 0.1 | 0.7 ± 0.1 | 0.8 ± 0.1 | 0.7 ± 0.1 |
| 54:5 | 2.1 ± 0.04 | 1.7 ± 0.1 | n.d. | n.d. | n.d. | n.d. |
| 54:6 | 1.4 ± 0.2 | 1.11 ± 0.04 | 2.5 ± 0.4 | 2.9 ± 0.3 | 2.4 ± 0.4 | 2.3 ± 0.2 |
| 54:7 | 0.21 ± 0.03 | 0.16 ± 0.02 | 1.0 ± 0.1 | 1.1 ± 0.1 | 1.1 ± 0.1 | 1.2 ± 0.1 |
| 56:0 | 1.0 ± 0.1 | 1.10 ± 0.02 | 0.06 ± 0.01 | 0.11 ± 0.04 | n.d. | 0.11 ± 0.02 |
| 56:1 | 2.0 ± 0.1 | 1.6 ± 0.1 | 0.14 ± 0.01 | 0.25 ± 0.03 | 0.12 ± 0.02 | 0.169 ± 0.004 |
| 56:2 | 1.9 ± 0.1 | 1.7 ± 0.1 | 0.23 ± 0.02 | 0.40 ± 0.1 | 0.23 ± 0.03 | 0.25 ± 0.02 |
| 56:3 | 1.50 ± 0.03 | 1.28 ± 0.03 | 0.20 ± 0.03 | 0.34 ± 0.04 | 0.32 ± 0.04 | 0.30 ± 0.01 |
| 56:6 | 3.9 ± 0.4 | 2.4 ± 0.04 | 4.0 ± 1.0 | 3.6 ± 0.4 | 5.0 ± 1.0 | 4.7 ± 0.3 |
| 56:7 | 1.7 ± 0.3 | 1.1 ± 0.1 | 5.0 ± 1.0 | 4.6 ± 0.3 | 6.9 ± 0.4 | 6.3 ± 0.4 |
| 58:1 | 1.91 ± 0.02 | 1.68 ± 0.04 | 0.11 ± 0.02 | 0.21 ± 0.04 | 0.13 ± 0.02 | 0.22 ± 0.04 |
| 58:2 | 1.2 ± 0.1 | 1.02 ± 0.01 | 0.12 ± 0.01 | 0.20 ± 0.02 | 0.12 ± 0.02 | 0.13 ± 0.01 |
| 58:3 | 0.62 ± 0.04 | 0.51 ± 0.03 | 0.06 ± 0.01 | 0.11 ± 0.01 | 0.11 ± 0.02 | 0.14 ± 0.04 |
| 58:5 | 1.7 ± 0.2 | 0.8 ± 0.1 | 0.3 ± 0.1 | 0.26 ± 0.02 | 0.7 ± 0.2 | n.d. |
| 58:6 | 3.0 ± 0.4 | 1.9 ± 0.3 | n.d. | n.d. | n.d. | n.d. |
| 58:7 | 2.1 ± 0.3 | 1.7 ± 0.4 | 4.9 ± 0.4 | 4.9 ± 0.2 | 7.0 ± 1.0 | 6.9 ± 0.3 |
| 58:8 | 0.9 ± 0.1 | 0.6 ± 0.2 | 3.4 ± 0.4 | 3.4 ± 0.2 | 5.2 ± 0.2 | 4.9 ± 0.1 |
| 58:9 | 0.2 ± 0.1 | 0.2 ± 0.1 | 1.0 ± 0.1 | 0.92 ± 0.01 | 1.8 ± 0.1 | 1.7 ± 0.1 |
| 58:10 | n.d. | 0.07 ± 0.03 | 0.82 ± 0.01 | 0.78 ± 0.02 | 1.267 ± 0.002 | 1.23 ± 0.01 |
| 58:11 | n.d. | n.d. | 0.9 ± 0.1 | 0.88 ± 0.03 | 1.1 ± 0.1 | 1.2 ± 0.1 |
| 58:12 | n.d. | 0.03 ± 0.01 | 2.7 ± 0.4 | 2.9 ± 0.3 | 2.0 ± 0.4 | 2.1 ± 0.1 |
| 60:1 | 2.3 ± 0.1 | 1.85 ± 0.02 | 0.13 ± 0.04 | 0.22 ± 0.03 | 0.15 ± 0.03 | 0.26 ± 0.03 |
| 60:2 | 1.50 ± 0.02 | 1.21 ± 0.02 | 0.14 ± 0.01 | 0.20 ± 0.02 | 0.16 ± 0.02 | 0.21 ± 0.04 |
| 60:3 | 0.60 ± 0.02 | 0.47 ± 0.02 | 0.05 ± 0.01 | 0.10 ± 0.02 | 0.09 ± 0.01 | 0.10 ± 0.02 |
| 60:4 | 0.4 ± 0.1 | 0.3 ± 0.10 | n.d. | 0.05 ± 0.01 | 0.09 ± 0.02 | 0.08 ± 0.01 |
| 60:10 | 0.1 ± 0.1 | 0.11 ± 0.04 | 1.9 ± 0.1 | 1.76 ± 0.01 | 3.1 ± 0.2 | 3.2 ± 0.1 |
| 60:12 | 1.1 ± 0.4 | 0.45 ± 0.03 | 18.7 ± 0.3 | 18.0 ± 1.0 | 15.0 ± 1.0 | 16.0 ± 0.4 |
| 60:13 | 0.1 ± 0.1 | 0.062 ± 0.004 | 3.0 ± 0.4 | 3.1 ± 0.1 | 2.0 ± 1.0 | 2.5 ± 0.1 |
| 62:1 | 1.0 ± 0.1 | 0.76 ± 0.03 | n.d. | 0.08 ± 0.01 | n.d. | 0.10 ± 0.01 |
| 62:2 | 0.9 ± 0.1 | 0.620 ± 0.003 | 0.06 ± 0.02 | 0.08 ± 0.01 | 0.08 ± 0.02 | 0.12 ± 0.01 |
| 62:3 | 0.43 ± 0.02 | 0.31 ± 0.01 | 0.04 ± 0.01 | 0.050 ± 0.004 | 0.07 ± 0.02 | 0.07 ± 0.01 |
| 62:11 | n.d. | 0.04 ± 0.01 | 1.0 ± 0.1 | n.d. | 1.0 ± 0.1 | 1.2 ± 0.1 |
| 62:12 | 0.32 ± 0.04 | 0.3 ± 0.1 | 8.7 ± 0.2 | 6.6 ± 0.4 | 8.2 ± 0.1 | 7.0 ± 1.0 |
| 62:13 | 0.5 ± 0.2 | 0.43 ± 0.01 | 17.3 ± 1.3 | 14.6 ± 1.1 | 15.59 ± 0.02 | 15.0 ± 1.0 |
| 62:14 | n.d. | n.d. | 2.7 ± 0.4 | 2.6 ± 0.2 | 2.2 ± 0.4 | 2.4 ± 0.2 |
| 66:18 | 0.18 ± 0.04 | 0.18 ± 0.01 | 12.3 ± 2.2 | 11.2 ± 1.4 | 7.0 ± 2.0 | 7.0 ± 1.0 |
a
The lipidomic data is presented as mean percentages of each species per total detected species and normalised to total cellular protein.
b
TAG species are presented according to the number of carbon atoms and double bonds.
c
MDA-MB-231 cells were reverse transfected with ATGL-targeting siRNA (siATGL) or non-targeting scrambled siRNA (SCR) and treated with 100 µM DHA and 10 nM sPLA2 in complete medium for 48 h. Cell lysates were collected for UPLC/MS analysis. Values are means ± SEM of three independent experiments; n.d., not determined.