Table 4.
PC profiles of MDA-MB-231 breast cancer cells depleted in ATGL and treated with exogenous DHA, sPLA2 or both.
|
PC composition (% of total)a |
||||||
|---|---|---|---|---|---|---|
| PC speciesb | SCRc | siATGL | SCR+DHA | siATGL+DHA | SCR+DHA+sPLA2 | siATGL+DHA+sPLA2 |
| 30:0 | 2.6 ± 0.3 | 2.7 ± 0.2 | 4.3 ± 0.1 | 5.7 ± 0.2 | 2.8 ± 0.1 | 4.7 ± 0.4 |
| 30:1 | 1.9 ± 0.3 | 2.2 ± 0.2 | 1.88 ± 0.04 | 2.44 ± 0.02 | 1.8 ± 0.1 | 2.3 ± 0.1 |
| 32:0 | 5.3 ± 0.2 | 5.3 ± 0.2 | 21.0 ± 1.0 | 25.0 ± 1.0 | 12.0 ± 1.0 | 12.2 ± 1.1 |
| 32:1 | 9.0 ± 1.0 | 9.5 ± 0.4 | 6.6 ± 0.2 | 7.3 ± 0.2 | 5.8 ± 0.1 | 6.3 ± 0.2 |
| 34:1 | 28.0 ± 1.0 | 28.8 ± 1.4 | 19.0 ± 1.0 | 18.0 ± 1.0 | 25.0 ± 1.0 | 21.2 ± 0.2 |
| 34:2 | 5.4 ± 0.2 | 4.9 ± 0.4 | 3.1 ± 0.1 | 2.9 ± 0.1 | 3.2 ± 0.1 | 2.6 ± 0.2 |
| 34:3 | 0.26 ± 0.02 | 0.28 ± 0.01 | 1.12 ± 0.01 | 1.2 ± 0.1 | 0.58 ± 0.03 | 0.6 ± 0.1 |
| 36:0 | 0.38 ± 0.01 | 0.5 ± 0.1 | 0.321 ± 0.001 | 0.29 ± 0.02 | 0.51 ± 0.03 | 0.5 ± 0.1 |
| 36:1 | 20.0 ± 1.0 | 21.8 ± 1.4 | 9.0 ± 0.1 | 6.0 ± 1.0 | 15.0 ± 1.0 | 13.6 ± 1.1 |
| 36:2 | 13.4 ± 0.3 | 12.8 ± 0.3 | 5.8 ± 0.1 | 4.3 ± 0.3 | 9.0 ± 1.0 | 6.3 ± 0.3 |
| 36:4 | 2.4 ± 0.1 | 2.4 ± 0.1 | 2.6 ± 0.1 | 2.8 ± 0.1 | 2.9 ± 0.2 | 2.8 ± 0.1 |
| 36:5 | 0.5 ± 0.1 | 0.26 ± 0.02 | 1.31 ± 0.03 | 1.8 ± 0.1 | 0.9 ± 0.1 | 1.52 ± 0.02 |
| 36:6 | 0.11 ± 0.02 | 0.04 ± 0.01 | 0.8 ± 0.1 | 0.8 ± 0.1 | 0.4 ± 0.1 | 0.39 ± 0.02 |
| 38:3 | 1.8 ± 0.1 | 1.6 ± 0.1 | 1.05 ± 0.01 | 0.8 ± 0.1 | 1.5 ± 0.1 | 1.33 ± 0.03 |
| 38:4 | 2.8 ± 0.4 | 2.4 ± 0.2 | 2.5 ± 0.1 | 1.9 ± 0.1 | 3.1 ± 0.2 | 3.0 ± 0.1 |
| 38:5 | 2.0 ± 1.0 | 1.3 ± 0.1 | 2.4 ± 0.4 | 1.9 ± 0.2 | 3.2 ± 0.2 | 3.1 ± 0.3 |
| 38:6 | 1.7 ± 0.3 | 0.8 ± 0.1 | 8.0 ± 1.0 | 9.0 ± 1.0 | 6.0 ± 1.0 | 7.9 ± 0.3 |
| 40:6 | 1.5 ± 0.2 | 1.2 ± 0.1 | 6.0 ± 1.0 | 6.0 ± 1.0 | 5. 0 ± 1.0 | 7.8 ± 0.3 |
| 40:7 | 1.0 ± 0.1 | 0.31 ± 0.01 | 3.0 ± 0.1 | 2.3 ± 0.2 | 2.5 ± 0.2 | 2.0 ± 0.1 |
a
The lipidomic data is presented as mean percentages of each species per total detected species and normalised to total cellular protein.
b
PC species are presented according to the number of carbon atoms and double bonds.
c
MDA-MB-231 cells were reverse transfected with ATGL-targeting siRNA (siATGL) or non-targeting scrambled siRNA (SCR) and treated with 100 µM DHA and 10 nM sPLA2 in complete medium for 48 h. Cell lysates were collected for UPLC/MS analysis. Values on the graphs are means ± SEM of three independent experiments; n.d., not determined.