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. 2018 Jun 5;9:1177. doi: 10.3389/fmicb.2018.01177

FIGURE 3.

FIGURE 3

Effects of preincubation of promastigotes with sCD100 on infection index. Peritoneal macrophages from BALB/c mice were infected with L. (L.) amazonensis at a MOI of 5 for 4 (A), 24 (B), 48 (C), or 72 (D) hours at different conditions: macrophages and parasites without sCD100 (control), promastigotes preincubated with sCD100 at a concentration of 200 ng/mL (promastigote+sCD100), macrophages and promastigotes in the continuous presence of sCD100 at a concentration of 200 ng/mL (sCD100). Statistical analysis was performed by ANOVA with Tukey’s post-test, and significant differences are labeled as ∗p ≤ 0.05. Results of a representative experiment of three.