Figure 8.

Determination of Pax7-positive satellite cells by IHC. (A) Tissue sections of female normal weight and obese C57BL/6J mice, labeled with anti-Pax7, and stained with DAB. Triangle = satellite cell. Pictures were taken with Olympus IX81 using Xcellence v.1.2. Scale = 50 μm at 40x magnification. (B) Calculated satellite cells per mm². Six animals per time point à three sections per animal were independently counted by three researchers, averaged and depicted. Black = normal weight; Gray = obese. Statistical analysis by homoscedastic two-sided t-test. ^*Indicates p ≤ 0.05.