Figure 6.

(A) Schematic of lentiviral vector with dual promoter, myc-hFTH, and GFP. (B) Analysis of in vitro MRI of agarose phantom suspended F-98 cells transfected with Lenti-myc-hFTH vector. (C) Prussian blue staining of iron deposits in mock and myc-hFTH tumors collected at 3 wk. (D) Immunofluorescence staining of myc-hFTH and GFP in mock and myc-hFTH tumors with anti-myc and anti-GFP antibodies. (E) In vivo FLI (left) and T2-weighted images (right) of MCF-7 tumors bearing myc-hFTH reporter gene after 21 days of subcutaneous transplantation. (A-E adapted with permission from ¹⁵⁰, copyright 2010 American Association for Cancer Research)