Figure 5.
5-HT6R-mediated RhoA GTPase signaling promotes bone destruction in ovariectomy-induced osteoporosis mice. (A) Scheme for the assessment of the ovariectomized (OVX) osteoporosis mouse model. (B-C) Serum 5-HT and TRAP were measured by ELISA. (D-G) μCT images are shown in distal femurs (D). Bone volume (BV) per tissue volume (TV) (E), trabecular number (Tb. N) (F), and trabecular thickness (Tb. Th) (G) were analyzed. (H-J) H&E staining was performed on the tibial and femurs sections (H). The number of osteoclasts (OCs) was analyzed in the tibial (I) and femurs sections (J). Scale bar: 100 μm. Rho: Rhosin. *, p < 0.05 compared to Ovx mice. #, p < 0.05 compared to Ovx + ST1936. Data shown represent mean ± SEM.