Table 3.
| Base d | Formula | Nucleotide Monomer
|
Polynucleotide Polymer c
|
|||
|---|---|---|---|---|---|---|
| Acid e | Base−2 f | Acid e | Base−1 f | Na+ Salt | ||
| A | C10H14O6N5P | 331.222(1) | 329.206(1) | 313.207(1) | 312.199(1) | 334.181(1) |
| T | C10H15O8N2P | 322.208(1) | 320.192(1) | 304.193(1) | 303.185(1) | 325.167(1) |
| G | C10H14O7N5P | 347.221(1) | 345.206(1) | 329.206(1) | 328.198(1) | 350.180(1) |
| C | C9H14O7N3P | 307.197(1) | 305.181(1) | 289.182(1) | 288.174(1) | 310.156(1) |
| Mean | 326.962(2) | 324.946(2) | 308.947(2) | 307.939(2) | 329.921(2) | |
| 30:30:20:20 Mean h | 326.913(2) | 324.897(2) | 308.898(2) | 307.890(2) | 329.872(2) | |
| AT g | C20H29O14N7P2 | 653.430(2) | 649.398(2) | 617.400(2) | 615.384(2) | 659.347(2) |
| GC g | C19H28O14N8P2 | 654.419(2) | 650.387(2) | 618.388(2) | 616.372(2) | 660.336(2) |
| Mean | 653.925(3) | 649.893(3) | 617.894(3) | 615.878(3) | 659.842(3) | |
| 60:40 Mean i | 653.826(3) | 649.794(3) | 617.795(3) | 615.779(3) | 659.743(3) | |
a
Ignoring end-groups and methylation
b
Molar masses are calculated from the atomic masses, uncertainties, and ranges listed in (Meija et al., 2016) [27]. Standard uncertainties, estimated following (Possolo et al., 2017) [28], are enclosed by parentheses
c
Condensation polymer; n-1 waters are lost for every n condensed monomers
d
A: deoxyadenosine monophosphate, T: deoxythymidine monophosphate, G: deoxyguanosine monophosphate, C: deoxycytidine monophosphate
e
Conjugate acid, phosphate oxygens fully protonated
f
Conjugate base, phosphate oxygens fully deprotonated
g
Base pairs (bp) associated via hydrogen bonding
h
Calculated as (30A + 30 T + 20G + 20C)/100
i
Calculated as (60AT + 40GC)/100