Fig. 1.

T cell proliferation is impaired by dexamethasone. Healthy donor T cells were cultured for four days with αCD3/CD80 microbeads in the presence of vehicle or dexamethasone. a Representative flow cytometry plots of CellTrace violet dilution. Plots were derived from gated CD4 (top row) or CD8 (bottom row) T cells. b Negatively-selected healthy donor T cells were stained and proliferation analyses determined by flow cytometry following four days of culture under the indicated conditions. Precursor Frequency, Expansion Index, and Proliferation Index are shown. Each symbol is the average of duplicate wells, and each paired symbol represents a different donor (n = 5 donors). Statistical significance was determined with a paired two-tailed T test. c Cell cycle analysis was performed on healthy donor T cells cultured with vehicle or dexamethasone and stimulated with αCD3/CD80 microbeads. EdU uptake and DNA content were used to identify G₀/G₁, S, and G₂/M phases. Representative flow images (top) and quantification of duplicate wells are shown (bottom) from two independent experiments. d Lysates from healthy donor T cells incubated with the indicated microbeads and vehicle or dexamethasone were probed for the indicated proteins. GAPDH was used as a loading control and is shown for each individual blot. Data are representative of three independent experiments