Fig. 6.

CTLA-4 blockade rescues lymphocyte defects induced by dexamethasone. GL261 ffluc-mCherry tumor-bearing mice were randomized into the indicated cohorts. Vehicle or dexamethasone treatment was initiated on day 7, and isotype or CTLA-4 blocking antibody were administered on days 13, 16, and 19 following tumor implantation. Mice were euthanized on day 23 and tissues were harvested for flow cytometry analysis. a-b CD4 (a) and CD8 (b) T cells were quantified along with the indicated differentiation subsets using CD44 and CD62L expression. Brains (n = 8) and cervical lymph nodes (n = 10) were collected. Data are analyzed using a unpaired students T test. c The relative contribution of each differentiation subset is shown for CD4 (top) and CD8 (bottom) TILs. d The total number of IFNγ-producing T cells were quantified from the tumor-bearing hemispheres of mice from the indicated cohorts. Data are analyzed using an unpaired students T test. N = 8 mice/group