Fig. 4.

SOD1 suppression by LCS-1 induces apoptosis and inhibits proliferation. a The viability of the indicated cells exposed to LCS-1 (24 h) was detected with a CCK-8 kit. b and c CCK-8 assays of 5-8F and CNE2 cells treated with LCS-1 (1 μM) at the indicated time points. d Images (left panel) and quantification (right panel) of the clonogenicity of 5-8F and CNE2 cells treated with LCS-1. e Cell apoptosis was measured by Annexin-V/PI assays in LCS-1-treated cells and control cells for 48 h. f Paraffin-embedded tumour sections from mice with CNE2 cell xenografts were stained with haematoxylin and eosin or SOD1 antibody. Apoptotic cells were visualized and quantified by TUNEL staining (green) and were counterstained with DAPI (blue). Scale bar: 50 μm. All error bars represent the S.D. of at least three replicates from two independent experiments. *p < 0.05, **p < 0.01, versus the corresponding control