Fig. 5.

SOD1 suppression disrupts ROS homeostasis. a and b O₂•- levels were measured by a superoxide kit in the indicated cells after treated by siRNAs for 24 h. c and d O₂•- levels were measured by a superoxide kit in the indicated cells after treated by LCS-1 for 12 h. e and f Increased O₂•- levels in the indicated cells treated with LCS-1 in comparison with those in the control cells, the flow cytometry analysis results are shown. g Quantification of clonogenicity of 5-8F cells in the indicated groups. h Apoptotic cells were visualized by TUNEL staining (green) and were counterstained with DAPI (blue). Scale bar: 100 μm. All error bars represent the S.D. of at least three replicates from two independent experiments.* p < 0.05 compared with the control