FIG 2.

Demonstration of broad-host-range applicability. We tested vancomycin susceptibility of L. plantarum BAA-793, L. fermentum ATCC 14931, L. brevis ATCC 8287, L. sakei ATCC 15521, and L. salivarius CCUG 47825 harboring pSIP-ddlF260Y_plantarum (pVPL3933) with (+) and without (−) induced expression of DdlF260Y_plantarum. The vancomycin concentrations used in this assay ranged from 0 to 1,000 μg/ml (left to right). Cultures were also supplemented with 5 μg/ml erythromycin to select for the presence of pSIP-ddlF260Y_plantarum. Data shown are representative of results from three biological replicates, with a maximum MIC variation of ±100 μg/ml.