FIG 6.

DdrI is required for the regulation of cell division and/or genomic DNA segregation. (A) Fluorescence microscopy analyses of the cell shape of exponential (A₆₅₀, 0.3), transitional (A₆₅₀, 1.5) and stationary (A₆₅₀, 3.5) phases cells from GY9613 (wild type) and GY13630 (ΔddrI mutant) strains. The percentage of cells found in dyads (2 cells, blue), tetrads (4 cells, red) and two-tetrads (8 cells, green) are illustrated in the pie charts. Two-tetrads are indicated by green arrows. (B) Membranes were stained with FM4-64 (red) and nucleoids with DAPI (green). Anucleate cells are indicated by yellow arrows. n represents the total number of cells examined.