FIG 2.

Protection against the antimicrobial activity of cationic NCR247 with extracellular addition of succinoglycan. Early-exponential-phase S. meliloti cells were treated for 5 h with 20 μM NCR247 and 10 μg ml⁻¹ of the defined succinoglycan preparations in MOPS-GS buffer with CAs, and then the number of viable cells was determined. Bars and error bars indicate the means ± standard deviations. The results shown are representative of trends observed in at least two independent experiments. SN, supernatant; wt, wild-type; EPS, exopolysaccharide (succinoglycan); NS, not significant. ***, P ≤ 0.001; **, P ≤ 0.01; *, P ≤ 0.05.