FIG 8.

Succinoglycan-modifying residues affecting polysaccharide binding to NCR247. Summary plots of biolayer interferometry experiments with succinoglycan preparations from S. meliloti mutants that are unable to modify succinoglycan with one of three modifications, i.e., succinyl (exoH), pyruvyl (exoV), or acetyl (exoZ). Octet biolayer interferometry assays were performed as described in Materials and Methods. The concentration of free succinoglycan was kept constant at 10 μg ml⁻¹ for all derivatives. Where indicated, the ammonium acetate assay buffer was supplemented with 0.1 M NaCl or the indicated amounts of ethanol. Results shown are representative of trends observed in at least two independent experiments. 3V, supernatant precipitated with 3 volumes of ethanol; 7V, 3-volume fraction precipitated with an additional 7 volumes of ethanol.