TABLE 2.
Sporulation efficiencies of B. subtilis strains
| Strain | Relevant genotype | Yield (%) of heat-resistant sporesa |
|---|---|---|
| 1A1 | Wild type | 67 ± 4b |
| LMD101 | ΔspoVD | 0c |
| LMD158 | ΔpbpI | 55 ± 9 |
| LMD162 | ΔspoVD ΔpbpI amyE::ery | 0c |
| LMD163 | ΔspoVD amyE::Pxyl-gfp-spoVD | 83 ± 5 |
| LMD166 | ΔspoVD ΔpbpI amyE::Pxyl-gfp-spoVD | 57 ± 2 |
| LMD167 | ΔspoVD ΔpbpI amyE::Pxyl-gfp-spoVD2–214-pbpI236–584 | 0c |
| LMD168 | ΔspoVD ΔpbpI amyE::Pxyl-gfp-pbpI1–235-spoVD215–646 | 0c |
| LMD170 | ΔspoVD ΔpbpI amyE::Pxyl-gfp-pbpI | 0c |
| LMD174 | ΔspoVD ΔpbpI amyE::Pxyl-gfp-pbpI1–40-spoVD41–646 | 0c |
| LMD175 | ΔspoVD ΔpbpI amyE::Pxyl-gfp-spoVD2–40-pbpI41–584 | 0c |
| LMD185 | ΔspoVD ΔpbpI amyE::PσE-gfp-spoVD | 68 ± 9d |
| LMD186 | ΔspoVD ΔpbpI amyE::PσE-gfp-pbpI | 0c,d |
| LMD187 | ΔspoVD ΔpbpI amyE::PσE-gfp-pbpI1–40-spoVD41–646 | 0c,d |
| LMD188 | ΔspoVD ΔpbpI amyE::PσE-gfp-spoVD2–40-pbpI41–584 | 0c,d |
a
Cells were sporulated for 2 days at 37°C in NSMP supplemented with 0.2% (wt/vol) xylose. The heat-resistant spore yield was assayed by incubation at 80°C for 10 min and calculated as CFU after heating divided by CFU of nonheated culture. The results are presented as the averages from three independent experiments ± SEMs.
b
Data from reference 15. The cells were grown in the absence of xylose.
c
No colonies were obtained when 100 μl heated culture was plated on TBAB. Three independent experiments were done, including one biological replicate using another clone.
d
The cells were grown in the absence of xylose.