TABLE 3.
Sporulation efficiencies of B. subtilis strains containing GFP-SpoVE-PBP4b or GFP-SpoVE-SpoVD triple-fusion protein
| Strain | Relevant genotype | Yield (%) of heat-resistant sporesa |
|---|---|---|
| LMD101 | ΔspoVD | 0b |
| LMD121 | ΔspoVE | 0b |
| LMD189 | ΔspoVD amyE::Pxyl-gfp-spoVE-spoVD | 87 ± 1 |
| LMD190 | ΔspoVD amyE::Pxyl-gfp-spoVE-pbpI | 0b |
| LMD193 | ΔspoVE amyE::Pxyl-gfp-spoVE-pbpI | 77 ± 4 |
| LMD194 | ΔspoVE amyE::Pxyl-gfp-spoVE-spoVD | 73 ± 1 |
a
Cells were sporulated for 2 days at 37°C in NSMP supplemented with 0.2% (wt/vol) xylose. The results are presented as the averages from three independent experiments ± SEMs.
b
No colonies were obtained when 100 μl heated culture was plated on TBAB. Three independent experiments were done, including one biological replicate using another clone.