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Traditional sequencing showing ongoing substitutions in Anabaena PCC 7120 substrain IHB. Sequencing was performed with PCR products generated using DNA extracted from substrain IHB or single colonies of IHB. (A) Sequencing before colony isolation. (B) Sequencing after colony isolation (the complementary strand compared to that in panel A). 1 to 6, DNA extracted with cells derived from 6 colonies.
