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. 2018 Apr 25;15(6):5231–5242. doi: 10.3892/etm.2018.6097

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Copyright: © Prompunt et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Cellular ROS levels following treatment of ARVMs with rhSLPI. ARVMs were incubated with carboxy-H2DCFDA and treated with 0, 200, 400, 600, 800 and 1,000 ng/ml rhSLPI. Following incubation, H₂O₂ was applied to each group and ROS production was determined using the EnSpire Multimode Plate Reader. Data are presented as the mean ± standard error of the mean (n=3). *P<0.05 vs. control group; ^#P<0.05 vs. H₂O₂ treated group. ROS, reactive oxygen species; ARVM, adult rat ventricular myocytes; rhSLPI, recombinant human secretory leukocyte protease inhibitor; A.U., arbitrary unit.