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. 2018 Apr 25;15(6):5231–5242. doi: 10.3892/etm.2018.6097

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Copyright: © Prompunt et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — The effect of rhSLPI treatment on the myocardial infarct size of ex vivo murine hearts during myocardial I/R. (A) The study protocol for all experimental groups. The hearts of adult male C57BL/6 mice were perfused for 40 min with modified Krebs solution, or with modified Krebs solution containing 400 ng/ml rhSLPI or 1,000 ng/ml rhSLPI. Hearts were then subjected to 30 min global ischemia and 2 h reperfusion. (B) Infarct size was determined by TTC staining. Each bar graph represents the mean ± standard error of the mean (n=6). *P<0.05 vs. I/R alone. rhSLPI, recombinant human secretory leukocyte protease inhibitor; I/R, ischemia/reperfusion; LV, left ventricle.