Figure 7.

Signaling in response to treatment of ex vivo murine hearts with rhSLPI during myocardial I/R. The hearts of adult male C57BL/6 mice were perfused for 40 min with modified Krebs solution or modified Krebs solution containing 400 ng/ml or 1,000 ng/ml rhSLPI followed by 10 min global ischemia. Hearts were homogenized with homogenizing buffer and protein was collected. The activation of (A) p38 MAPK and (B) Akt was assessed using western blot analysis. Each bar graph represents the fold phosphorylation of p38 MAPK and Akt. *P<0.05 vs. I/R control group (n=3). rhSLPI, recombinant human secretory leukocyte protease inhibitor; MAPK, mitogen-activated protein kinase; p-, phosphorylated; t-, total; I/R, ischemia/reperfusion.