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. 2018 Apr 27;15(6):5205–5214. doi: 10.3892/etm.2018.6107

Figure 5.

Figure 5.

Effect of SIRT1 upregulation on miR-142-3p-mediated suppression of proliferation and chemoresistance of ovarian cancer cells. miR-142-3p-overexpressing SKOV3 cells were transfected with SIRT1 expression plasmid or a blank vector. Post-transfection, (A) RT-qPCR and (B) western blotting were used to detect the mRNA and protein expression of SIRT1. (C) An MTT assay was used to examine cell proliferation. Subsequently, miR-142-3p-overexpressing SKOV3/DDP cells were transfected with a SIRT1 expression plasmid or a blank vector. Post-transfection, (D) RT-qPCR and (E) western blotting was used to detect the mRNA and protein expression levels of SIRT1, and (F) a Cell Counting kit-8 assay was used to examine drug sensitivity. **P<0.01 vs. miR1423p + blank. miR, microRNA; blank, blank vector; SIRT1, sirtuin 1; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.