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. 2018 May 3;15(6):5251–5260. doi: 10.3892/etm.2018.6125

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Copyright: © Song et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effect of HSYA on apoptosis levels and ROS generation in MSCs. (A) Determination of cell apoptosis through fluorescence staining procedures following exposure to H/SD treatment for 48 h (scale bar, 50 µm). (B) Quantitative MSC apoptosis levels at 6, 12, 24 and 48 h. (C) Determination of ROS accumulation through fluorescence staining following exposure to H/SD treatment for 48 h (scale bar, 50 µm), and (D) determination of DCF fluorescence intensity by FCM expressed relative to the normal control group following 6–48 h of H/SD treatment. (E) Quantitative ROS levels at 6, 12, 24 and 48 h. (F) Western blotting analysis of cytC and caspase-3 following 24 h of H/SD culture. (G) Quantification of relative band intensities. ^##P<0.01 vs. the normal. **P<0.01 vs. the H/SD group. HSYA, hydroxysafflor yellow A; ROS, reactive oxygen species; MSCs, mesenchymal stem cells; H/SD, hypoxia and serum deprivation; FCM, flow cytometry; DCF, 2′,7′-dichlorodihydrofluorescein diacetate; Cyto.cytC, cytoplasmic cytochrome C; C.caspsase-3, cleaved caspase-3.