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Reverse transcription-quantitative polymerase chain reaction primer sequences.
| Genes | Primers (5′-3′) | Tm (°C) | Fragment length (bp) |
|---|---|---|---|
| HIF-1α | 55 | 218 | |
| Sense | AACAAACAGAATCTGTCCTCAAACC | ||
| Antisense | CAGGTAATGGAGACATTGCCAG | ||
| VEGF | 55 | 151 | |
| Sense | CAGCGACAAGGCAGACTATT | ||
| Antisense | GTTGGCACGATTTAAGAGGG | ||
| β-actin | 55 | 246 | |
| Sense | TCACCCACACTGTGCCCATCTATGA | ||
| Antisense | CATCGGAACCGCTCATTGCCGATAG |
HIF-α, hypoxia-inducible factor-α; VEGF, vascular endothelial growth factor; Tm, annealing temperature; bp, base pairs.
