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. Author manuscript; available in PMC: 2019 Apr 2.
Published in final edited form as: Curr Biol. 2018 Mar 22;28(7):1150–1156.e4. doi: 10.1016/j.cub.2018.02.046

Figure 2. Ccnb3 knockdown results in precocious gene expression.

Figure 2

(A) In situ hybridizations of Foxa.a in early Ciona embryos injected with MOs targeting Hes.a, Ccnb3, and Pem individually or in combination.

(B) In situ hybridizations of Tfap2-r.b under the same experimental conditions as A.

(C) In situ hybridizations of Foxd.b with BIO treatments and Ccnb3 or Ccnb3 + Pem targeting MO injections. Expression in cells matching controls are indicated with a white arrowhead. The expansion of expression into additional cells is indicated with a yellow arrowhead. Precocious expression is indicated with a red arrowhead. Embryos are orientated anterior left. Scale bar = 50 µm. See also Figures S2–S3.