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. Author manuscript; available in PMC: 2018 Jun 12.
Published in final edited form as: Bioconjug Chem. 2018 Jan 17;29(2):296–305. doi: 10.1021/acs.bioconjchem.7b00622

Figure 1.

Figure 1

Stability of polyplexes and conjugate polyplexes against disassembly. (a) Heparin-induced siRNA release from the polyplexes and conjugate polyplexes prepared at w/w ratio = 2 and 5 in 5 mM HEPES buffer (pH 7.4) and incubated with increasing concentrations of heparin with (+) or without (−) 20 mM DTT. (b) Stability of the polyplexes and conjugate polyplexes in 50% FBS and PBS following incubation for 0–24 h. The release of free siRNA in both (a) and (b) was visualized by agarose gel electrophoresis. (c) Free siRNA degradation profile in 50% FBS.