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. Author manuscript; available in PMC: 2018 Jun 12.
Published in final edited form as: Proteomics. 2017 Mar;17(6):10.1002/pmic.201600335. doi: 10.1002/pmic.201600335

Figure 2. Representative Hematoxylin and Eosin Stained Tissue Sections for Good Quality and Challenging Tissue Specimens and Relative Quantification of Tissue Quality Control Proteins.

Figure 2

Frozen sections from tumors have been selected to illustrate high cellularity tumor tissue (A) as well as samples with ascites (B), necrosis (C), and blood (D). In panel E, albumin and hemoglobin are measured with LC-SRM to define tissues that may include ascites or whole blood, which contributes significantly to the total protein amount derived from the tumor tissue. Samples with high levels of these proteins, which could disrupt the biomarker measurements, are marked with red arrows. Cellular markers include lactate dehydrogenases, actin, heat shock cognate 71, nuclear lamin, GAPDH, vimentin, and enolase. Samples with low amounts of these cellular proteins are marked with black arrows to indicate samples that may lack sufficient cellularity for IP-LC-SRM of bulk tumor tissue. The scale bar for relative expression is shown (right).