ALG3 regulates cellular proliferation, migration, and invasion. (A) ALG3 was down-regulated by infection with LV3-shALG3-1 or LV3-shALG3-2. (B) Breast cancer MCF-7 cells were infected with LV3-NC, LV3-shALG3-1, or LV3-shALG3-2. Cell proliferation was assessed by CCK-8. (C, D) Breast cancer MCF-7 cell migration ability was detected by the wound healing assay. The migration of LV3-shALG3-1- or LV3-shALG3-2-infected MCF-7 cells was lower compared to LV3-NC-infected cells. (E, F) Breast cancer MCF-7 cell invasion ability was detected by Matrigel invasion assays. The invasion ability of LV3-shALG3-1- or LV3-shALG3-2-infected MCF-7 cells was decreased compared with LV3-NC-infected cells. Original magnification, 200×. Error bars represent standard error. * p<0.05, and **p<0.001.