Table 1.
Sclerotinia sclerotiorum (Lib) de Bary population used for studying the virulence and genetic diversity within isolates based on morphological features, SSR profiling, and ITS sequence analysis.
| Strain No. | Source (Host) | Geographic origin | Latitude and Longitude | Province | Disease severity observed in Brassica cultivars (Lesion length cm) | ||||
|---|---|---|---|---|---|---|---|---|---|
| B. juncea (NRCDR-2) | B. rapa var Toria (Uttara) | B. rapa var yellow sarson (NRCYS 5-2) | B. rapa var brown sarson | Mean | |||||
| ESR 1 | B. juncea | DRMR, Bharatpur | 27°15′N; 77°30′E | Rajasthan | 14.0 | 18.7 | 21.0 | 17.0 | 17.7 |
| ESR 2 | B. juncea | Oraiya | 26°46′N; 79°51′ E | UP | 13.7 | 13.0 | 18.7 | 13.0 | 14.6 |
| ESR 3 | B. rapa | Imlia, Varansai | 26°4′N; 77°9′E | UP | 15.7 | 13.7 | 20.0 | 14.7 | 16.0 |
| ESR 4 | B. juncea | CSA, Kanpur | 26°28′N; 80° 24′E | UP | 6.5 | 8.0 | 8.0 | 8.3 | 7.7 |
| ESR 5 | B.rapa | Allahbad | 25°28′N; 81° 54′E | UP | 12.5 | 16.0 | 17.3 | 12.0 | 14.5 |
| ESR 6 | B. juncea | BHU, Varanasi | 25°26′N; 82°9′E | UP | 12.0 | 10.0 | 18.7 | 16.7 | 14.4 |
| ESR 7 | B. juncea | Akbarpur, Kanpur | 26°26′N; 52° 33′E | UP | 14.7 | 13.0 | 23.0 | 12.7 | 15.9 |
| ESR 8 | B. juncea | Etawah | 26°47′N; 79° 02′E | UP | 5.7 | 12.7 | 16.7 | 10.0 | 11.3 |
| ESR 9 | B. juncea | Firozabad | 27°09′N; 78° 24′E | UP | 14.0 | 18.3 | 25.0 | 14.3 | 17.9 |
| ESR 10 | B. juncea | Khaga | 25°47′N; 81°07′E | UP | 9.7 | 8.7 | 13.7 | 8.7 | 10.2 |
| ESR 11 | B. juncea | Kokhraj | 26°55′N; 80°59′E | UP | 5.0 | 4.7 | 6.3 | 4.7 | 5.2 |
| ESR 12 | B. juncea | Koshambi | 25°48′N; 81°66′E | UP | 16.7 | 14.0 | 22.7 | 15.0 | 17.1 |
| ESR 13 | B. rapa | Meghipur | 24°94′N; 85.69 E | Bihar | 11.7 | 13.7 | 19.3 | 14.3 | 14.8 |
| ESR 14 | B. juncea | Morena | 26°49′N; 77°76′E | MP | 8.0 | 6.3 | 8.0 | 4.7 | 6.8 |
| ESR 15 | B. juncea | Handia | 25°38′N; 82°18′E | UP | 19.0 | 24.3 | 25.0 | 21.0 | 22.3 |
| ESR 16 | B. juncea | Bawal | 28°08′N; 76°58′E | Haryana | 14.0 | 13.3 | 18.0 | 19.0 | 16.1 |
| ESR 17 | B. juncea | Maluka | 30°21′N; 74°96′E | Punjab | 17.7 | 17.0 | 24.0 | 20.7 | 19.9 |
| ESR 18 | B. juncea | Bhatinda | 30°11′N; 75°00′E | Punjab | 27.7 | 22.7 | 32.7 | 20.0 | 25.8 |
| ESR 19 | B. juncea | Moga | 30°48′N; 75°10′E | Punjab | 16.0 | 14.0 | 21.7 | 11.3 | 15.8 |
| ESR 20 | B. juncea | PAU, Ludhiana | 30°55′N; 75°54′E | Punjab | 15.0 | 13.3 | 25.7 | 14.0 | 17.0 |
| ESR 21 | B. juncea | Faridkot | 30°67′N; 74°73′E | Punjab | 11.3 | 15.0 | 19.3 | 10.0 | 13.9 |
| ESR 22 | B. juncea | Mahalkalan, Sangrur | 30°75′N; 76°78′E | Punjab | 13.7 | 16.3 | 20.7 | 17.3 | 17.0 |
| ESR 23 | B. juncea | Rajoana, Ludhiana | 30°72′N; 75°6′E | Punjab | 8.0 | 15.0 | 22.7 | 12.7 | 14.6 |
| ESR 24 | B. juncea | Ranchi | 23°23′N; 85° 23′E | Jharkhand | 4.3 | 4.0 | 9.0 | 4.0 | 5.3 |
| ESR 25 | B. juncea | Mathura | 27°28′N; 77°41′E | UP | 19.7 | 26.0 | 28.7 | 24.7 | 24.8 |
| ESR 26 | B. juncea | CCSHAU Hisar | 29°14′N; 75°72′E | Haryana | 13.0 | 16.0 | 20.0 | 17.0 | 16.5 |
| ESR 27 | B. juncea | Sirsa | 29°53′N; 75°01′E | Haryana | 14.0 | 12.3 | 20.0 | 14.0 | 15.1 |
| ESR 28 | B. juncea | Bassi, Jaipur | 26°91′N; 75°78′E | Rajasthan | 5.3 | 4.0 | 9.0 | 4.3 | 5.7 |
| ESR 29 | B. juncea | Shahganj, Janunpur | 24°70′N; 82°95′E | UP | 6.0 | 11.7 | 18.7 | 10.7 | 11.8 |
| ESR 30 | B. juncea | Jammu | 31°14′29″N; 77°2′12″E | J & K | 14.3 | 18.0 | 25.3 | 18.7 | 19.1 |
| ESR 31 | B. juncea | Bansur, Alwar | 27°71′N; 76°28′E | Rajasthan | 15.3 | 15.0 | 21.7 | 15.3 | 16.8 |
| ESR 32 | B. juncea | Badodamev, Alwar | 27°34′N; 76° 38′E | Rajasthan | 13.7 | 12.3 | 23.7 | 9.7 | 14.9 |
| ESR 33 | B. juncea | DU, SC | 28°36′N; 77°22′E | Delhi | 11.7 | 16.0 | 18.0 | 14.3 | 15.0 |
| ESR 34 | B. juncea | Pantnagar | 29°02′N; 79°48′E | Uttarakhand | 11.0 | 15.0 | 15.7 | 10.0 | 12.9 |
| ESR 35 | B. juncea | Kherli | 27°20′N; 77°03′E | Rajasthan | 23.7 | 23.3 | 31.3 | 22.0 | 25.1 |
| ESR 36 | B. juncea | Amoli | 27°09′N; 77°08′E | Rajasthan | 49.0 | 46.3 | 55.0 | 42.3 | 48.2 |
| ESR 37 | B. juncea | Dausa | 26°89′N; 76°33′E | Rajasthan | 11.5 | 11.3 | 19.0 | 12.0 | 13.5 |
| ESR 38 | B. juncea | Nadbai | 27°21′N; 77°20′E | Rajasthan | 4.3 | 14.7 | 14.7 | 13.3 | 11.8 |
| ESR 39 | B. juncea | Ludhawai | 27°21′N; 77°2′E | Rajasthan | 11.3 | 18.7 | 21.3 | 14.0 | 16.3 |
| ESR 40 | B. juncea | Sangaria,SGN | 29°79′N; 74°46′E | Rajasthan | 5.7 | 12.7 | 16.7 | 10.0 | 11.3 |
| ESR 41 | B. juncea | Sri Ganga nagar | 29°90′N; 73°87′E | Rajasthan | 8.0 | 12.7 | 16.7 | 10.0 | 11.9 |
| ESR 42 | B. juncea | Kumher | 27°34′N; 77°37′E | Rajasthan | 5.3 | 7.0 | 13.0 | 10.0 | 8.8 |
| ESR 43 | B. juncea | Gajsinghpur | 29°65′N; 73°43′E | Rajasthan | 8.3 | 16.0 | 17.7 | 12.0 | 13.5 |
| ESR 44 | B. juncea | Halena | 27°10′N; 77°15′E | Rajasthan | 8.0 | 11.7 | 15.3 | 10.0 | 11.3 |
| ESR 45 | B. juncea | Bajoli | 26°86′N; 74°4′E | Rajasthan | 9.7 | 18.3 | 15.3 | 13.3 | 14.2 |
| ESR 46 | B. juncea | Mahua | 27°05′N; 76°92′E | Rajasthan | 7.3 | 12.3 | 17.0 | 15.3 | 13.0 |
| ESR 47 | B. juncea | Kiratpura, Jaipur | 31°18′N; 76°.56′E | Punjab | 4.7 | 11.0 | 11.3 | 12.7 | 9.9 |
| ESR 48 | B. juncea | Pathredi | 27°59′N; 76°09′E | Rajasthan | 8.7 | 10.7 | 12.0 | 11.0 | 10.6 |
| ESR 49 | B. juncea | Bhadubha | 26°89′N; 75°81′E | Rajasthan | 4.3 | 13.7 | 14.0 | 14.0 | 11.5 |
| ESR 50 | B. juncea | Tulsipura | 27°57′N; 82°45′E | UP | 2.0 | 14.0 | 12.0 | 11.3 | 9.8 |
| ESR 51 | B. juncea | Chhonkarwara | 27°10′N; 77°04′E | Rajasthan | 9.7 | 14.3 | 19.7 | 14.3 | 14.5 |
| ESR 52 | B. juncea | Kaimasi | 27°08′N; 77°38′E | Rajasthan | 5.0 | 14.3 | 16.0 | 10.7 | 11.5 |
| ESR 53 | B. juncea | Habibpur | 25°21′N; 86°98′E | UP | 1.7 | 12.3 | 12.3 | 12.3 | 9.7 |
| ESR 54 | B. juncea | Baansi | 24°32′N; 74°38′E | Rajasthan | 2.3 | 6.3 | 10.0 | 10.7 | 7.3 |
| ESR 55 | B. juncea | Jhalatala | 27°08′N; 77°01′E | Rajasthan | 1.7 | 16.0 | 14.7 | 11.7 | 11.0 |
| ESR 56 | B. juncea | Gundwa | 27°24′N; 77°46′E | Rajasthan | 1.3 | 2.0 | 7.3 | 4.3 | 3.7 |
| ESR 57 | B. juncea | Deeg | 27°34′N; 77°37′E | Rajasthan | 4.0 | 1.3 | 4.7 | 2.7 | 3.2 |
| ESR 58 | B. juncea | Sewar | 27°18′N; 77°44′E | Rajasthan | 8.0 | 12.0 | 13.0 | 8.7 | 10.4 |
| ESR 59 | B. juncea | Hantra | 27°12′N; 77°24′E | Rajasthan | 2.7 | 4.3 | 6.0 | 4.0 | 4.3 |
| ESR 60 | B. juncea | Kanma | 27°08′N; 77°1′E | Rajasthan | 2.0 | 10.0 | 13.0 | 9.0 | 8.5 |
| ESR 61 | B. juncea | Baansi | 24°32′N; 74°38′E | Rajasthan | 12.7 | 11.3 | 18.7 | 11.7 | 13.6 |
| ESR 62 | B. juncea | Dehra mod | 27°21′N; 77°49′E | Rajasthan | 2.0 | 2.0 | 6.0 | 4.7 | 3.7 |
| ESR 63 | B. juncea | Aroda | 23°75′N; 72°89′E | Rajasthan | 15.0 | 15.0 | 25.7 | 15.0 | 17.7 |
| ESR 64 | B. juncea | Bhandor | 27°26′N; 77°45′E | Haryana | 6.0 | 8.7 | 12.3 | 11.0 | 9.5 |
| ESR 65 | B. juncea | Basua | 25°02′N; 87°32′E | Rajasthan | 16.0 | 18.0 | 26.0 | 18.0 | 19.5 |
The severity of disease assessed on Brassica cultivars was measured in lesion length (cm). Disease index for the strains listed, accessed on length of the lesion developed on stem (in cm). Lesion size 0.0 cm: Non-virulent; 0.1–3.0 cm: less virulent; 3.1–10.0: Moderately virulent; 10.1–20.0 cm: virulent; >20.0 cm: highly virulent. NRCDR-02 stands for National Research Centre Disease Resistance-02, YS stands for yellow sarson