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. 2018 Apr 25;9:732. doi: 10.3389/fimmu.2018.00732

Figure 4.

Figure 4

Neutrophil elastase (NE) degrades TNF, IL-6, and IL-8 (A). THP-1-derived macrophages were exposed to hNE (125–500 mU/mL) in the presence or absence of HK-Spn or LPS (100 ng/mL) for 4 h. TNF, IL-6, and IL-8 concentrations in the culture supernatants were determined by ELISA. Data represent the mean ± SD of quadruplicate experiments and were evaluated using one-way analysis of variance with Tukey’s multiple-comparisons test. *Significantly different within the same activation status at P < 0.05. (B) rhTNF, rhIL-6, and rhIL-8 were exposed to hNE (125–500 mU/mL) in the presence or absence of SSH (100 µg/mL) for 3 h and determined by western blot analysis. ELISA, enzyme-linked immunosorbent assay; HK-Spn, heat-killed S. pneumoniae; LPS, lipopolysaccharide; hNE, human neutrophil elastase; rh, recombinant human; SSH, sivelestat sodium hydrate; IL, interleukin; TNF, tumor necrosis factor.