Table 1.
no LPS | LPS | Fixed effect, p-values | Contrasts, p-values2 | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
DON (mg/kg feed) | 0 | 2.5 | 5 | 10 | 0 | 2.5 | 5 | 10 | SEM | DON | LPS | DON × LPS | 0 vs. DON | lin. | quad. |
Clostridiales family 1 | 34.99 | 39.00 | 31.83 | 47.66 | 29.74 | 37.48 | 39.09 | 45.33 | 8.39 | 0.38 | 0.94 | 0.89 | 0.26 | 0.14 | 0.66 |
Ruminococcaceae | 31.12 | 32.97 | 31.30 | 30.09 | 34.06 | 28.87 | 25.32 | 30.85 | 4.55 | 0.82 | 0.62 | 0.74 | 0.47 | 0.54 | 0.55 |
Enterobacteriaceae | 15.44 | 12.49 | 12.43 | 8.88 | 14.61 | 15.44 | 17.36 | 8.63 | 3.06 | 0.14 | 0.43 | 0.76 | 0.32 | 0.07 | 0.24 |
Turicibacteraceae | 9.51 | 7.09 | 10.90 | 5.68 | 11.58 | 9.61 | 8.75 | 6.93 | 3.75 | 0.69 | 0.73 | 0.92 | 0.44 | 0.35 | 0.80 |
Lachnospiraceae | 5.71 | 6.84 | 10.88 | 6.03 | 7.57 | 6.58 | 7.07 | 5.59 | 1.78 | 0.33 | 0.60 | 0.46 | 0.72 | 0.97 | 0.20 |
Lactobacillaceae | 0.58 | 0.45 | 0.47 | 0.37 | 0.44 | 0.90 | 0.60 | 0.28 | 0.22 | 0.48 | 0.59 | 0.55 | 0.97 | 0.34 | 0.23 |
kingdom unassigned | 0.61 | 0.30 | 0.30 | 0.35 | 0.33 | 0.39 | 0.32 | 0.37 | 0.07 | 0.13 | 0.46 | 0.06 | 0.02 | 0.11 | 0.08 |
Clostridiaceae | 0.47 | 0.14 | 0.25 | 0.14 | 0.32 | 0.11 | 0.07 | 0.14 | 0.08 | <0.01 | 0.12 | 0.66 | <0.01 | 0.01 | 0.03 |
Clostridiales family 2 | 0.15 | 0.18 | 0.15 | 0.20 | 0.11 | 0.12 | 0.12 | 0.17 | 0.02 | 0.08 | 0.03 | 0.97 | 0.14 | 0.04 | 0.38 |
Coriobacteriaceae | 0.11 | 0.10 | 0.07 | 0.14 | 0.10 | 0.21 | 0.09 | 0.15 | 0.05 | 0.31 | 0.33 | 0.54 | 0.58 | 0.80 | 0.83 |
Erysipelotrichaceae | 0.13 | 0.06 | 0.06 | 0.07 | 0.11 | 0.11 | 0.08 | 0.15 | 0.04 | 0.60 | 0.32 | 0.67 | 0.34 | 0.72 | 0.20 |
Corynebacteriaceae | 0.00 | 0.28 | 0.06 | 0.10 | 0.11 | 0.03 | 0.03 | 0.03 | 0.08 | 0.52 | 0.26 | 0.17 | 0.60 | 0.79 | 0.51 |
Enterococcaceae | 0.02 | 0.04 | 0.05 | 0.01 | 0.02 | 0.02 | 0.08 | 0.02 | 0.02 | 0.14 | 0.74 | 0.79 | 0.42 | 0.77 | 0.09 |
Peptostreptococcaceae | 0.02 | 0.02 | 0.02 | 0.01 | 0.03 | 0.03 | 0.04 | 0.01 | 0.01 | 0.14 | 0.16 | 0.69 | 0.46 | 0.29 | 0.25 |
Data are presented as least squares means ± standard error of the mean (SEM); n = 10 per treatment group.
P-values for orthogonal contrasts to test linear (lin.) and quadratic (quad.) relationships between control feeding and the 3 increasing levels of DON as well as the overall difference of 0 DON vs. all DON groups (0 vs. DON).