. 2018 Apr 25;9:804. doi: 10.3389/fmicb.2018.00804

Table 1.

Relative abundance (%) of families in cecal digesta of broiler chickens fed diets with increasing levels of deoxynivalenol (DON; 0, 2.5, 5, or 10 mg DON/kg diet) and with or without oral lipopolysaccharide challenge (LPS) 1 day prior slaughter¹.

	no LPS				LPS					Fixed effect, p-values			Contrasts, p-values²
DON (mg/kg feed)	0	2.5	5	10	0	2.5	5	10	SEM	DON	LPS	DON × LPS	0 vs. DON	lin.	quad.
Clostridiales family 1	34.99	39.00	31.83	47.66	29.74	37.48	39.09	45.33	8.39	0.38	0.94	0.89	0.26	0.14	0.66
Ruminococcaceae	31.12	32.97	31.30	30.09	34.06	28.87	25.32	30.85	4.55	0.82	0.62	0.74	0.47	0.54	0.55
Enterobacteriaceae	15.44	12.49	12.43	8.88	14.61	15.44	17.36	8.63	3.06	0.14	0.43	0.76	0.32	0.07	0.24
Turicibacteraceae	9.51	7.09	10.90	5.68	11.58	9.61	8.75	6.93	3.75	0.69	0.73	0.92	0.44	0.35	0.80
Lachnospiraceae	5.71	6.84	10.88	6.03	7.57	6.58	7.07	5.59	1.78	0.33	0.60	0.46	0.72	0.97	0.20
Lactobacillaceae	0.58	0.45	0.47	0.37	0.44	0.90	0.60	0.28	0.22	0.48	0.59	0.55	0.97	0.34	0.23
kingdom unassigned	0.61	0.30	0.30	0.35	0.33	0.39	0.32	0.37	0.07	0.13	0.46	0.06	0.02	0.11	0.08
Clostridiaceae	0.47	0.14	0.25	0.14	0.32	0.11	0.07	0.14	0.08	<0.01	0.12	0.66	<0.01	0.01	0.03
Clostridiales family 2	0.15	0.18	0.15	0.20	0.11	0.12	0.12	0.17	0.02	0.08	0.03	0.97	0.14	0.04	0.38
Coriobacteriaceae	0.11	0.10	0.07	0.14	0.10	0.21	0.09	0.15	0.05	0.31	0.33	0.54	0.58	0.80	0.83
Erysipelotrichaceae	0.13	0.06	0.06	0.07	0.11	0.11	0.08	0.15	0.04	0.60	0.32	0.67	0.34	0.72	0.20
Corynebacteriaceae	0.00	0.28	0.06	0.10	0.11	0.03	0.03	0.03	0.08	0.52	0.26	0.17	0.60	0.79	0.51
Enterococcaceae	0.02	0.04	0.05	0.01	0.02	0.02	0.08	0.02	0.02	0.14	0.74	0.79	0.42	0.77	0.09
Peptostreptococcaceae	0.02	0.02	0.02	0.01	0.03	0.03	0.04	0.01	0.01	0.14	0.16	0.69	0.46	0.29	0.25

Data are presented as least squares means ± standard error of the mean (SEM); n = 10 per treatment group.

P-values for orthogonal contrasts to test linear (lin.) and quadratic (quad.) relationships between control feeding and the 3 increasing levels of DON as well as the overall difference of 0 DON vs. all DON groups (0 vs. DON).