Table 2.

Differences in relative abundance (%) of genera in cecal digesta of broiler chickens fed diets with increasing levels of deoxynivalenol (DON; 0, 2.5, 5, or 10 mg DON/kg diet) and with or without oral lipopolysaccharide challenge (LPS) 1 day prior slaughter¹.

	no LPS				LPS					Fixed effect, p-values			Contrasts, p-values2
DON (mg/kg feed)	0	2.5	5	10	0	2.5	5	10	SEM	DON	LPS	DON × LPS	0 vs. DON	lin.	quad.
Enterobacteriaceae genus 1	15.24	11.94	10.80	8.14	14.18	14.41	16.52	8.51	2.84	0.12	0.35	0.66	0.20	0.04	0.35
Oscillospira	3.16	1.81	2.84	2.09	2.71	1.59	2.23	2.74	0.35	0.01	0.52	0.28	0.01	0.51	0.03
Anaerotruncus	1.04	1.32	2.51	1.44	1.47	1.44	2.80	1.32	0.51	0.02	0.62	0.95	0.19	0.31	0.05
Blautia	0.33	0.76	1.23	0.54	0.54	0.99	0.86	0.72	0.21	0.03	0.69	0.43	0.02	0.26	0.01
unassigned bacterial phylum	0.61	0.30	0.30	0.35	0.33	0.39	0.32	0.37	0.07	0.13	0.46	0.06	0.02	0.11	0.08
Clostridiales genus 2	0.15	0.18	0.15	0.20	0.11	0.12	0.12	0.17	0.02	0.08	0.03	0.97	0.14	0.04	0.38
Clostridiaceae genus	0.28	0.06	0.14	0.08	0.13	0.04	0.02	0.07	0.08	0.18	0.17	0.73	0.03	0.15	0.16
Clostridium	0.20	0.08	0.10	0.06	0.19	0.07	0.05	0.06	0.03	<0.01	0.42	0.78	<0.01	<0.01	0.01
Dorea	0.07b	0.08b	0.18a	0.08b	0.14ab	0.11ab	0.08b	0.07b	0.03	0.15	0.94	0.01	0.81	0.42	0.16
Enterobacteriacee genus 2	0.015	0.013	0.016	0.036	0.010	0.016	0.017	0.019	0.007	0.14	0.38	0.50	0.22	0.04	0.36
Ruminococcaceae genus 2	0.010	0.005	0.003	0.003	0.008	0.006	0.002	0.004	0.002	0.05	0.87	0.84	0.01	0.02	0.15

¹Data are presented as least squares means ± standard error of the mean (SEM); n = 10 per treatment group; only values for genera that were different (p < 0.05) are presented.

²P-values for orthogonal contrasts to test linear (lin.) and quadratic (quad.) relationships between control feeding and the 3 increasing levels of DON as well as the overall difference of 0 DON vs. all DON groups (0 vs. DON).

^{a, b}DON × LPS interaction: Least squares means of genera with no common superscripts differ significantly between groups; p < 0.05.