. 2018 Apr 25;9:804. doi: 10.3389/fmicb.2018.00804

Table 3.

Differences in relative abundance (%) of COG pathways (Cluster of orthologous groups of proteins) in cecal digesta of broiler chickens fed diets with increasing levels of deoxynivalenol (DON; 0, 2.5, 5, or 10 mg DON/kg diet) and with or without oral lipopolysaccharide challenge (LPS) 1 day prior slaughter¹.

	no LPS				LPS					Fixed effect, p-values			Contrasts, p-values²
DON (mg/kg feed)	0	2.5	5	10	0	2.5	5	10	SEM	DON	LPS	DON × LPS	0 vs. DON	lin.	quad.
CELLULAR PROCESSES
Cell motility	2.94	3.47	3.20	3.68	2.98	3.42	3.44	3.67	0.27	0.08	0.77	0.95	0.02	0.02	0.73
Transport and catabolism	0.18	0.17	0.17	0.17	0.18	0.17	0.16	0.17	0.01	0.10	0.70	0.67	0.02	0.05	0.13
METABOLISM
Metabolism of cofactors and vitamins	3.87	4.05	4.00	4.08	3.92	4.02	4.01	4.05	0.07	0.08	0.96	0.94	0.01	0.03	0.41
Lipid metabolism	2.92	2.87	2.89	2.82	2.93	2.86	2.85	2.81	0.04	0.12	0.73	0.97	0.05	0.03	0.96
Enzyme families	2.13	2.11	2.12	2.11	2.14	2.10	2.10	2.11	0.01	0.13	0.54	0.70	0.02	0.09	0.12
Glycan biosynthesis and metabolism	1.68	1.62	1.61	1.58	1.68	1.67	1.65	1.59	0.04	0.16	0.40	0.92	0.09	0.03	0.84
Metabolism of terpenoids and polyketides	1.58	1.53	1.54	1.51	1.55	1.53	1.53	1.51	0.02	0.05	0.58	0.83	0.01	0.02	0.60
Biosynthesis of other secondary metabolites	0.74	0.79	0.79	0.79	0.77	0.78	0.77	0.79	0.01	<0.01	0.88	0.09	<0.01	<0.01	0.37
UNCLASSIFIED
Poorly characterized	5.00	4.84	4.88	4.79	4.97	4.93	4.92	4.82	0.07	0.10	0.51	0.88	0.04	0.02	0.93
Genetic information processing	2.78^a	2.70^bc	2.69^b	2.71^bc	2.73^abc	2.76^ac	2.73^abc	2.71^bc	0.02	0.08	0.43	0.04	0.02	0.02	0.37

Data are presented as least squares means ± standard error of the mean (SEM); n = 10 per treatment group; only values for COG pathways that were different (p < 0.05) are presented.

P-values for orthogonal contrasts to test linear (lin.) and quadratic (quad.) relationships between control feeding and the three increasing levels of DON as well as the overall difference of 0 DON vs. all DON groups (0 vs. DON).

^{a, b, c}

DON × LPS interaction: Least squares means of COG pathways with no common superscripts differ significantly between groups; p < 0.05.