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. 2018 Apr 25;9:552. doi: 10.3389/fpls.2018.00552

FIGURE 6.

FIGURE 6

Immunofluorescence localization of JIM11 epitope during symbiotic seed germination of D. officinale. (A) The uncolonized embryo with strong signals of JIM11 epitope (green color, excitation 488 nm and emission 500–530 nm). The signals (arrows) are present mainly in the walls of the middle and basal regions of the embryo. The orange color indicates the autofluorescence (excitation 488 nm and emission 565–615 nm). Scale bar = 50 μm. (B) After 1 week of inoculation, the infected embryo showing strong signals of JIM11 epitope (arrows) in the walls of the middle and basal regions of the swollen embryo. Scale bar = 50 μm. (C) After 2 weeks of inoculation, the enlarged embryo with the rupture of the seed coat showing prominent signals of JIM 11 epitope in the pelotons (arrowheads) and the walls of colonized cells (arrows) in the basal protocorm. Scale bar = 50 μm. (D) After 3 weeks of inoculation, the signals of JIM 11 epitope (arrows) are mainly observed in the walls of colonized cells of the developing protocorm. Scale bar = 100 μm.