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. 2018 Apr 25;9:552. doi: 10.3389/fpls.2018.00552

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Copyright © 2018 Li, Chen, Zhang, Cho, Wang, Yeung, Zeng, Guo and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunogold staining of JIM11 epitope in the symbiotic protocorms of D. officinale. (A) In the apical portion of the protocorm, no immunogold particles were observed. Scale bar = 1 μm. (B) TEM of a basal portion of protocorm cell with fungal hyphae (H). Labeling occurred in the host cell wall (CW) and the interfacial matrix (arrowhead) or fungal cell wall. Scale bar = 1 μm. (C) TEM of a portion of protocorm cell with collapsed fungal hyphae (H). Scale bar = 1 μm. (D) The control staining. Thin sections were incubated with the mouse pre-immune IgG instead of JIM11 antibody. Immunogold particles were absent in these treatments. Scale bar = 1 μm.