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. 2018 Apr 25;9:552. doi: 10.3389/fpls.2018.00552

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Copyright © 2018 Li, Chen, Zhang, Cho, Wang, Yeung, Zeng, Guo and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of 3,4-DHP on the localization of JIM11 epitope during symbiotic seed germination of D. officinale. (A) After 3 weeks of inoculation, most embryos did not enlarge, and weak signals of JIM11 epitope (arrow) are observed in the walls of basal end of the embryo. Scale bar = 50 μm. (B) After 3 weeks of inoculation, the infected embryo showing weak signals of JIM11 epitope (arrow) in the walls of basal end of the swollen embryo. Scale bar = 50 μm. (C) Most seeds in the treatment with 3,4-DHP are unable to germinate, and the severe fungal invasion in the ungerminated seeds could be commonly observed. Scale bar = 50 μm.