Table 2.
The intensity evaluation of immunofluorescence labeling with JIM11 antibody during the formation of protocorm of D. officinale in symbiotic culture.
| Developmental stages | Embryo regions | JIM11 signal intensity |
|---|---|---|
| Stage 0 | Apical region | - |
| Middle region | ++ | |
| Basal region | ++ | |
| Stage 1 | Apical region | - |
| Middle region | +++ | |
| Basal region | ++++ | |
| Stage 2 | Apical region | - |
| Middle region | + | |
| Basal region | +++ | |
| Stage 3 | Apical region | - |
| Middle region | + | |
| Basal region | +++ | |
Increasing intensity was evaluated as: - (no signal); ±(very weak); + (weak); ++ (intermediate); +++ (strong); ++++ (very strong).