Table 1.
Panels of antibodies used for flow cytometry staining.
| Specificity | Clone | Flurochrome of the Ab in the mix for |
||
|---|---|---|---|---|
| Skin cells | LN cells | Blood | ||
| CD1a | O10 | AF700 | AF700 | N/A |
| HLA-DR | G46-6 | APC-Cy7 | APC-Cy7 | V500 |
| CD163 | GHI/61 | BV711 | BV711 | N/A |
| CD11c | 3.9 | BV510 | BV510 | PE-Cy7 |
| CD45 | DO58-1283 | PerCp | N/A | N/A |
| CD123 | 7G3 | N/A | PercP | PercP |
| CD66abce | TET2 | APC | APC | FITC |
| CD3 | SP34-2 | V450 | V450 | APC-Cy7 |
| CD8 | RPA-T8 | N/A | N/A | BV650 |
| CD20 | 2H7 | V450 | V450 | APC-Cy7 |
| CD11b | Bear 1 | PE-Cy7 | PE-Cy7 | A700 |
| CD16 | 3G8 | ECD | ECD | APC |
| NKG2a | Z199 | PE | PE | PE |
| CD14 | M5E2 | N/A | N/A | V450 |
| CD33 | AC104.3E3 | N/A | N/A | PE |