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. 2018 Apr 25;9:807. doi: 10.3389/fmicb.2018.00807

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Copyright © 2018 Shao, Abdel-Motaal, Chen, Chen, Xu, Meng, Zhang, Meng and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mapping of the inserted DNA fragment in the recombinant plasmid pUC-SL38. One 5′-end truncated ORF1 designated SppA and one intact ORF2 designated RDD are included in a 1145-bp DNA fragment (GenBank accession No. KY575967) inserted into the recombinant plasmid pUC-SL38, the latter of which is preceded by a respective promoter-like sequence and a respective Shine–Dalgarno (SD) sequence. The predicted promoter sequence (-35 region and -10 region), the SD sequence, the ribosomal binding site (RBS) and the initiation codon ATG of RDD are underlined. The transcription initiation site (TIS) is highlighted in bold and also marked with the leftward arrow. The stop codon TAA of ORF2 is indicated by the asterisk and a possible terminator following RDD indicated by the inverted solid arrows.