FIGURE 10.

Effect of RDD variants by site-directed mutagenesis on bacterial growth. To identify the roles of the target residues on the protein function of RDD, E. coli KNabc expressing wild-type RDD or each of its all variants were grown in the LBK media at pH 7.0 containing no addition of NaCl or LiCl (A), 0.2 M NaCl (B) or 5 mM LiCl (C), or at pH 8.0 with the addition of 50 mM NaCl (D) or at pH 8.5 without the addition of NaCl (E). The above mentioned cell growth was ended after 24 h and the values for OD_{600 nm} then evaluated. Each data point represents the average of three independent determinations.